| 溶藻细菌H5的分离、鉴定及溶藻特性研究 |
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| 引用本文： | 刘国勇,胡亚平,石小丹,聂小倩,黄应平.溶藻细菌H5的分离、鉴定及溶藻特性研究[J].安徽农业科学,2012,40(28):13955-13956,13959. |
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| 作者姓名： | 刘国勇 胡亚平 石小丹 聂小倩 黄应平 |
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| 作者单位： | 1. 三峡大学三峡库区生态环境教育部工程研究中心,湖北宜昌,443002
2. 中国长江三峡集团公司中华鲟研究所,湖北宜昌,443100 |
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| 基金项目： | 国家自然科学基金项目(50979049,51179096);三峡大学博士启动基金项目(KJ2008B016) |
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| 摘 要： | 目的]分离和鉴定溶藻细菌,研究其溶藻特性,为进一步研究溶藻细菌对水华的治理作用提供帮助。方法]从香溪河春季水华集聚区水体中分离得到1株有高效溶藻效果的菌株(H5),采用16S rDNA序列相似性分析和Biolog微生物自动鉴定系统等对细菌进行鉴定。采用直接计数法,研究了其对汉斯冠盘藻(Stephanodiscus hantzschii)、倪氏拟多甲藻(Peridiniopsis niei)、具尾逗隐藻(Komma cau-data)的抑制效果,及对汉斯冠盘藻的溶藻作用方式。结果]根据生理生化及16S rDNA序列分析鉴定,H5属于纺缍形赖氨酸芽孢杆菌(Lysinibacillus fusiformis)。该菌对汉斯冠盘藻、倪氏拟多甲藻、具尾逗隐藻的溶藻率最高为71.3%,最低为57.4%。培养滤液、热处理培养滤液对汉斯冠盘藻的生长具有明显抑制作用,而细菌无细胞提取物无溶藻能力。结论]该菌对汉斯冠盘藻有较强的溶藻效果,且是通过分泌溶藻物质溶藻。
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| 关 键 词： | 溶藻细菌 芽孢杆菌 水华 汉斯冠盘藻 香溪河 |
Isolation,Identifcation and Characterization of Algae-lysing Strain H5 from Xiangxi Bay of Three Gorges Reservoir |
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| Institution: | LIU Guo-yong et al(Engineering Research Center of Eco-environment in Three Gorges Reservoir Region,Ministry of Education,China Three Gorges University,Yichang,Hubei 443002) |
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| Abstract: | Objective] The study was conducted to isolate and identify alage-lysing bacterium and explore the characterization of alage-lysing bacterium so as to provide reference for the control of algae bloom.Method] A high-efficient algae-lysing strain H5 was isolated during spring blooms in Xiangxi Bay at Three Gorges Reservoir.According to the similarity analysis of its 16S rRNA gene sequence and by Biolog microstation system,the strain was identified.By direct counting,the lytic efficiency of H5 to Stephanodiscus hantzschii,Peridiniopsis niei,Komma caudata and performing mode of H5 to S.hantzschii were studied.Result] 16S rDNA sequence analysis and Biolog analysis showed that H5 strain belonged to Lysinibacillus fusiformis.The highest and lowest algicidal rate of the strain was 71.3% and 57.4% respectively in the cultures of S.hantzschii,P.niei,and K.caudata.The filtrate of cell spent media,and the heated above filtrate displayed the same algae-lytic ability,while the cell-free supernatant of the cells of bacteria showed no algae-lytic ability,indicating that some extracellular and thermo-stable substances were produced by this strain.Conclusion] The strain H5 produced better lytic efficiency to S.hantzschii and performed algae-lytic ability by producing extracellular substances. |
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| Keywords: | Algae-lysing bacterium Lysinibacillus fusiformis Bloom Stephanodiscus hantzschii Xiangxi River |
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