| 水稻PIN家族的生物信息学分析 |
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| 引用本文： | 丁懿,石彩娟,王万军.水稻PIN家族的生物信息学分析[J].安徽农业科学,2012(27):13238-13242. |
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| 作者姓名： | 丁懿 石彩娟 王万军 |
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| 作者单位： | 西南交通大学生命科学与工程学院 |
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| 摘 要： | 作为植物中最重要的生长素外输载体,PIN家族在各种植物中都拥有众多成员。对水稻基因组的BLAST搜索获得了12个PIN家族成员,分析发现:水稻PIN基因不均衡地分布在基因组染色体上;其内含子、外显子结构类似;PIN蛋白质拥有典型的载体蛋白序列特征,即亲/疏水性反复变化,存在多次跨膜结构域;几乎所有PIN蛋白都有一个NPNXY的内化结构域和7个功能未知的基序;PIN在早期即分化为两组成员,主要区别在于中间亲水环的部分缺失;并且在后来发生了支系特异的复制事件,从而形成了众多旁系同源基因。
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| 关 键 词： | PIN家族 水稻 生物信息学 |
Bioinformatics Analysis of PIN-formed Family in Oryza sativa |
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| Institution: | DING Yi et al(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031) |
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| Abstract: | PIN family is the most important auxin efflux carrier in plants,which encompasses lots of members in nearly all the higher plants.It turns out that there are as many as 12 PIN genes in Oryza sativa when we perform BLAST search against its genome.Our studies show that PIN genes in Oryza sativa are asymmetrically distributed;The exon-intron structure of the PIN genes are much the same in Oryza sativa;PIN proteins have all the necessary elements for acting as carrier proteins: the hydrophobicity profile,transmembrane domain;Motif analysis suggests that there are NPNXY motifs,a well characterized IM motif,and 7 other uncharacterized motif in almost all the PIN proteins;Neighbour-joing phylogenetic trees show that PINs family split into two major families in early stages,which can be distinguished by the length of hydrogen loop in the center of proteins,and later lineage-specific duplication appeared in Oryza sativa,resulting a bunch of paralogous in the genome of Oryza sativa. |
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| Keywords: | PIN family Oryza sativa Bioinformatics |
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