| 利用绒山羊原始生殖细胞培养胚胎干细胞的研究 |
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| 引用本文： | 图雅,卢玲.利用绒山羊原始生殖细胞培养胚胎干细胞的研究[J].安徽农业科学,2012(31):15274-15276. |
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| 作者姓名： | 图雅 卢玲 |
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| 作者单位： | 包头师范学院生物科学与技术学院,内蒙古包头,014030 |
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| 基金项目： | 包头师范学院校内项目(BSY2010-23) |
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| 摘 要： | 目的]建立绒山羊胚胎干细胞体系。方法]采用与其胎儿成纤维细胞共培养的方式从20 mm绒山羊胎儿生殖嵴及其周围组织分离克隆出大量原始生殖细胞(PGCS)集落。结果]这些集落的细胞经过多次传代具有胚胎干细胞(ES)的诸多特征,如鸟巢状结构、AKP染色阳性、具有连续传代能力等。结论]该细胞具有多能性,类似ES细胞。
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| 关 键 词： | 绒山羊 原始生殖细胞 胚胎干细胞 |
Study on Embryonic Stem Cell from Cultured Primordial Germ Cells of Down Producing Goat |
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| Institution: | TU Ya et al(College of Biologic Science and Technology,Baotou Normal University,Baotou,Inner Mongolia 014030) |
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| Abstract: | Objective] To establish embryonic stem cell system of goats.Method] Numerous primordial germ cells colonies were derived from gonadal ridge and the surrounding tissues 20 millimeter fetuses of down producing goat.Primordial germ cells and goats embryonic fibroblasts obtained from a conceptus of equivalent gestational age were co-cultured.Result] The colonies had some characteristics of embryonic stem cells,such as the morphology of nest-like,they continued to be AKP positive and the ability to be continuously passed.Conclusion] These cells were pluripotent and ES-like cells. |
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| Keywords: | Down producing goat Primordial germ cell Embryonic stem cells |
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