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| 地鳖虫多糖提取工艺的优化研究 | |
| 引用本文： | 罗志文,刘娟,张聪颖,赵永勋.地鳖虫多糖提取工艺的优化研究[J].安徽农业科学,2012,40(28):13978-13979,13982. |
| 作者姓名： | 罗志文 刘娟 张聪颖 赵永勋 |
| 作者单位： | 1. 佳木斯大学生命科学学院,黑龙江佳木斯154007;佳木斯大学药学院,黑龙江佳木斯154007 2. 佳木斯大学药学院,黑龙江佳木斯,154007 3. 佳木斯大学生命科学学院,黑龙江佳木斯,154007 |
| 基金项目： | 黑龙江省教育厅科学技术项目(11531384) |
| 摘 要： | 目的]通过试验来提取地鳖虫多糖,用于中药多糖的开发研究。方法]采用热水浸提法对地鳖虫多糖进行提取试验,采用单因素试验来选择浸提条件,以浸提时间、料液比、浸提温度、浸提次数这4个因素做正交试验,用苯酚-硫酸进行多糖含量测定。结果]浸提温度对地鳖虫多糖含量影响最大,其次为浸提次数,浸提时间和料液比影响较小。地鳖虫多糖最佳的提取工艺条件为提取时间1.5h,料液比1∶10 g/ml,浸提温度80℃,浸提次数3次。结论]影响昆虫水溶性多糖提取率的因素主要有浸提温度、浸提时间、浸提次数和料液比等。随着提取时间的延长,细胞破碎越完全,多糖溶出的越多,所得多糖含量则越高。 |
| 关 键 词： | 地鳖虫多糖 热水浸提法 提取工艺 提取条件优化 |
Optimization of the Extraction Technology of Eupolyphaga sinensis Polysaccharide |
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| Institution: | LUO Zhi-wen et al(College of Life Sciences,Jiamusi University,Jiamusi,Heilongjiang 154007) |
| Abstract: | Objective]To extract Eupolyphaga sinensis polysaccharide through experiment for the research and development of polysaccharides in traditional Chinese medicine.Method] The Eupolyphaga sinensis polysaccharides were extracted by hot water,and the extraction conditions were determined by single factor test,an orthogonal experiment was carried out with the four factors of extraction time,solid-liquid ratio,extraction temperature and extraction times,the polysaccharides content was determined using phenol-sulfuric acid.Result]Extraction temperature had the largest impact on the extraction of Eupolyphaga sinensis polysaccharides,then followed by extraction time,extraction time and solid-liquid ratio.The optimal extraction conditions were 1.5 h of extraction time,1∶ 10(g/ml) of solid-liquid ratio,80 ℃ of extraction temperature,and three times of extraction.Conclusion]The main factors influencing the extraction of insect water soluble polysaccharides included extraction temperature,extraction time,extraction times and solid-liquid ratio.With the prolonging extraction time,the cells disrupted completely,more polysaccharide was dissolved out,and the polysaccharides content improved. |
| Keywords: | Eupolyphaga sinensis polysaccharide Hot water extraction Extraction process Optimization of extraction conditions |
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