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山莓的多倍体诱导
引用本文:汤访评,宋春涛,陈晓艳.山莓的多倍体诱导[J].安徽农业科学,2012,40(29):14181-14182,14241.
作者姓名:汤访评  宋春涛  陈晓艳
作者单位:1. 绍兴文理学院生命科学学院,浙江绍兴,312000
2. 绍兴市林木种苗管理站,浙江绍兴,312000
摘    要:目的]研究用秋水仙素诱导山莓多倍体的方法。方法]诱导出的以山莓组培苗腋芽为试验材料,研究在不同秋水仙素浓度下山莓多倍体的诱导效果,并对诱导出的多倍体进行细胞学鉴定。结果]秋水仙素对山莓有严重的毒害作用,且其随浓度的增加山莓死亡率逐渐升高;多倍体诱导率随秋水仙素浓度的增加呈先升高后降低的趋势。结论]秋水仙素浓度为0.40‰时,山莓诱导多倍体效果最好。

关 键 词:山莓  组织培养  秋水仙素  多倍体

Polyploidy Induction of Rubus corchorifolius
Institution:TANG Fang-ping et al(College of Life Sciences,Shaoxing University,Shaoxing,Zhejiang 312000)
Abstract:Objective]To study the polyploidy induction method of Rubus corchorifolius by colchicines.Method]With the raspberry seedlings with axillary bud as the experimental material,the polyploidy was inducted with different colchicines concentrations and then cytologically identified.Result]Colchicine had serious adverse effects to Rubus corchorifolius,and the death rate of Rubus corchorifolius gradually improved with increasing concentration of colchicines;polyploid induction rate increased at first and then decreased with the improving colchicine concentration.Conclusion]The highest induction frequently was achieved with colchicines at 0.04‰.
Keywords:Rubus corchorifolius  Tissue culture  Colchicines  Polyploidy
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