| 恩诺沙星人工抗原及多克隆抗体的制备 |
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| 引用本文： | 邹晓楠,龚云飞,奚茜,李沐洁,张晓峰,方结红,陈宗伦,王唯芬,张明洲.恩诺沙星人工抗原及多克隆抗体的制备[J].安徽农业科学,2012(27):13415-13417. |
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| 作者姓名： | 邹晓楠 龚云飞 奚茜 李沐洁 张晓峰 方结红 陈宗伦 王唯芬 张明洲 |
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| 作者单位： | 1. 中国计量学院生命科学学院,浙江省生物计量与检验检疫技术重点实验室,浙江杭州310018
2. 浙江出入境检验检疫局技术中心,浙江杭州,310012 |
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| 基金项目： | 浙江省重点科技创新团队项目(2010R50028);浙江省科技成果转化项目(2011E61018);浙江省国境安全检验检疫科技创新服务平台项目(2006C17014) |
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| 摘 要： | 目的]制备恩诺沙星人工抗原及多克隆抗体。方法]采用活性酯法合成恩诺沙星的人工抗原,并通过紫外光谱扫描和SDS-PAGE电泳对其进行鉴定。利用免疫原(ENR-BSA)免疫新西兰大白兔,制备抗恩诺沙星的高亲和力和高特异性的多克隆抗体。结果]恩诺沙星成功地偶联到载体蛋白上。通过动物免疫,获得了高效价的抗血清,最高效价达到1∶100 000,说明人工抗原成功地诱导机体产生免疫应答。结论]恩诺沙星的人工抗原合成路线简单易行,可为进一步建立恩诺沙星的免疫检测方法奠定了基础。
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| 关 键 词： | 恩诺沙星 人工抗原 鉴定 多克隆抗体 |
Preparation of Artificial Antigen and Polyclonal Antibody of Enrofloxacin |
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| Institution: | ZOU Xiao-nan et al(College of Life Sciences of China Jiliang University,Zhejiang Provincial Key Laboratory of Bio-metrology,Inspection and Quarantine,Hangzhou,Zhejiang 310018) |
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| Abstract: | Objective] The research aimed to prepare the artificial antigens and polyclonal antibody of enrofloxain.Method] The artificial antigen of enrofloxacin was synthesized by using active ester method and it was identified by using UV wavelength scanning and SDS-PAGE.ENR-BSA was used to immune New Zealand white rabbits and prepare polyclonal antibody of enrofloxain with high affinity and high specificity.Result] Enrofloxacin was successfully coupled with carrier protein.High-titer antiserum was obtained after immunization and the highest titer reached 1∶100 000,which indicated that artificial antigens successfully induced the immune response.Conclusion] The synthesis route of ENR artificial antigen was simple and feasible.This research could lay the foundation for further establishment of the immune detection method of enrofloxacin. |
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| Keywords: | Enrofloxacin Artifical antigen Identification Polyclonal antibody |
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