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| 转基因玉米的定性PCR检测 | |
| 引用本文： | 袁磊,赵蕾,孙红炜,颜世磊,路兴波.转基因玉米的定性PCR检测[J].山东农业科学,2009(11):8-10. |
| 作者姓名： | 袁磊 赵蕾 孙红炜 颜世磊 路兴波 |
| 作者单位： | 1. 山东师范大学生命科学学院,山东,济南,250014;山东省农业科学院植物保护研究所,山东,济南,250100 2. 山东师范大学生命科学学院,山东,济南,250014 3. 山东省农业科学院植物保护研究所,山东,济南,250100 |
| 基金项目： | 农业部转基因重大专项，山东省农业科学院博士科研启动基金 |
| 摘 要： | 根据玉米内参照基因zSSIIb和转基因玉米中常见的CaMV35S、NOS、Bt、hpt等基因的特异性结构,设计5对定性PCR引物,经定性PCR检测,可以准确地将转基因玉米的目的基因检出,说明这5对引物具有较高的特异性和准确性。由此建立了一套转基因玉米定性PCR检测方法,用于转基因玉米及其产品的筛查、抽检。 |
| 关 键 词： | 转基因玉米 定性PCR zSSIIb基因 CaMV35S基因 NOS基因 Bt基因 hpt基因 |
Qualitative PCR Detection of Transgenic Maize |
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| YUAN Lei,ZHAO Lei,SUN Hong-Wei,YAN Shi-lei,LU Xing-Bo.Qualitative PCR Detection of Transgenic Maize[J].Shandong Agricultural Sciences,2009(11):8-10. | |
| Authors: | YUAN Lei ZHAO Lei SUN Hong-Wei YAN Shi-lei LU Xing-Bo |
| Institution: | YUAN Lei, ZHAO Lei ,SUN Hong - Wei, YAN Shi - lei ,LU Xing - Bo ( 1. College of Life Science, Shandong Normal University, Jinan 250014, China; 2. Institute of Plant Proteetion, Shandong Aeademy of Agricultural Sciences, Jinan 250100, China) |
| Abstract: | Based on the specific structures of maize endogenous reference gene zSSIIb and the general genes in transgenic maize,such as CaMV35S promoter gene,NOS gene,Bt gene and hpt gene,5 pairs of primers were designed for qualitative PCR and then the target DNA sequences specific to each transgenic maize could be detected,which showed that the primers were specific and exact.In addition,a qualitative PCR method was developed,by which the specific fragments from transgenic maize could be identified and detected. |
| Keywords: | Transgenic maize Qualitative PCR zSSIIb gene CaMV35S gene NOS gene Bt gene hpt gene |
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