| 可去除选择标记的转CpTI基因抗虫玉米研究 |
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| 引用本文： | 于海清, 渠柏艳, 付凤玲, 李晚忱.可去除选择标记的转CpTI基因抗虫玉米研究[J].玉米科学,2005,13(3):044-046. |
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| 作者姓名： | 于海清 渠柏艳 付凤玲 李晚忱 |
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| 作者单位： | 四川农业大学玉米研究所,四川,雅安,625014 |
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| 基金项目： | 国家自然科学基金项目(30070476) |
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| 摘 要： | 将组成型表达的玉米泛素启动子与豇豆胰蛋白酶抑制剂基因CpTI连接,插入根癌农杆菌双T-DNA质粒,构建一个T-DNA结构域含有抗潮霉素选择标记基因hyg;另一个T-DNA结构域含有抗虫基因的双T-DNA单子叶植物表达载体,用以转化农杆菌菌株,再通过共培养转化玉米胚性愈伤组织。通过潮霉素培养基抗性筛选,用特异PCR扩增和Southern杂交检测,从分化再生的T0代植株中,鉴定出7个转化CpTI基因的阳性植株。目前,正结合进行田间分离纯合和DNA分子鉴定,培育去除选择标记基因的转基因抗虫玉米自交系。
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| 关 键 词： | 玉米 转基因 抗虫 选择标记 CpTI |
| 文章编号： | 1005-0906（2005）03-0044-03 |
| 收稿时间： | 9/3/2004 12:00:00 AM |
| 修稿时间： | 2004-09-032004-11-01 |
Study on CpTI Transgenic Insect Resistant Maize with Removable Selective Marker |
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| YU Hai-qing; QU Bai-yan; FU Feng-ling; LI Wan-chen.Study on CpTI Transgenic Insect Resistant Maize with Removable Selective Marker[J].Journal of Maize Sciences,2005,13(3):044-046. |
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| Authors: | YU Hai-qing; QU Bai-yan; FU Feng-ling; LI Wan-chen |
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| Abstract: | Cowpea trypsin inhibitor gene(CpTI),promoted by ubiquitin promoter of constitutive expression,was inserted into double T-DNA plasmid of Agrobacterium tumefactions.Monocotyledonous expression vector of double T-DNA was constructed with selective marker gene hyg(hygromycin resistance) in one T-DNA structural domain and insect resistant gene in another T-DNA domain.Agrobacterium strain was transformed with this vector and used to transform embryonic callus of maize by co-culture.Seven CpTI transgenic plants were identified from differentiated and regenerated T0 population through resistant selection on hygromycin medium and molecular detection of specific PCR amplification and Southern hybridization.At present,inbred lines of transgenic insect resistant maize with selective marker gene removed are in separating and inbreeding program assisted by DNA marker detection. |
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| Keywords: | Maize Transgene Insect resistance Selective marker CpTI |
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