| 湖南省猬迭宫绦虫的线粒体nad5和rrnS基因的序列测定及种系发育分析 |
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| 引用本文： | 刘自逵,赵光辉,刘国华,宋海燕,李芬,刘毅.湖南省猬迭宫绦虫的线粒体nad5和rrnS基因的序列测定及种系发育分析[J].中国兽医学报,2012,32(2):258-261. |
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| 作者姓名： | 刘自逵 赵光辉 刘国华 宋海燕 李芬 刘毅 |
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| 作者单位： | 1. 湖南农业大学动物医学学院,湖南长沙,410128
2. 西北农林科技大学动物医学学院,陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金资助项目(30771616);长沙科技计划资助项目(K0902144-21) |
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| 摘 要： | 本研究旨在阐明猬迭宫绦虫湖南分离株线粒体烟酰胺腺嘌呤二核苷酸（NADH）脱氢酶亚单位5基因（nad5）部分序列（pnad5）和核糖体小亚基基因（rrnS）部分序列（prrnS）的遗传变异情况,并用pnad5和prrnS序列重构猬迭宫绦虫与其他绦虫的种群遗传关系。利用聚合酶链反应（PCR）扩增猬迭宫绦虫的pnad5和prrnS,应用ClustalX 1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mega4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树。结果显示,所获得的pnad5和prrnS序列与预期（片段的）长度一致,分别为531bp和328bp。种系发育树表明,湖南分离株与已知猬迭宫绦虫位于同一分枝。结果表明,由于猬迭宫绦虫pnad5和prrnS序列种内相对保守,种间差异较大,均可作为种间遗传变异研究的标记。
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| 关 键 词： | 猬迭宫绦虫 线粒体DNA nad5基因 rrnS基因 种系发育关系 |
Sequence and phylogenetic analysis of mitochondrial nad5 and rrnS genes for Spirometra erinaceieuropaei isolates from Hunan province |
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| LIU Zi-kui,ZHAO Guang-hui,LIU Guo-hua,SONG Hai-yan,LI Fen,LIU Yi.Sequence and phylogenetic analysis of mitochondrial nad5 and rrnS genes for Spirometra erinaceieuropaei isolates from Hunan province[J].Chinese Journal of Veterinary Science,2012,32(2):258-261. |
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| Authors: | LIU Zi-kui ZHAO Guang-hui LIU Guo-hua SONG Hai-yan LI Fen LIU Yi |
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| Institution: | 1*(1.College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;2.College of Veterinary Medicine,Northwest Science and Technology University of Agriculture and Forestry,Yangling,Shaanxi 712100,China) |
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| Abstract: | The objectives of the present study were to examine sequence variation in the mitochondrial NADHdehydrogenase subunit 5(nad5) gene and small subunit of ribosomal RNA(rrnS) gene among Spirometra erinaceieuropaei isolates from Hunan province,and to reconstruct their phylogenetic relationship using nad5 and rrnS sequences.The partial nad5(pnad5) and rrnS(prrnS) were amplified from each S.erinaceieuropaei sample,and pnad5 and prrnS sequences were aligned using the Clustal X 1.81.MP and NJ trees of pnad5 and prrnS were constructed using the software Phylip 3.67 version 4.0 and Mega version 4.0,and ML tree was also constructed using Puzzle version 5.2.The results showed that the lengths of pnad5 and prrnS sequences were 531 bp and 328 bp,respectively.The constructed phylogenetic tree revealed that the Hunan isolates and the S.erinaceieuropaei available in GenBank were clustered in the same clade.There is no significant variation in pnad5 and prrnS sequences within S.erinaceieuropaei,while inter-species difference is obvious.It is concluded that pnad5 and prrnS sequences can be used as genetic marker for population genetic studies of cestodes. |
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| Keywords: | Spirometra erinaceieuropaei mitochondrial DNA nad5 gene rrnS gene phylogenetic relationship |
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