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| 不同大麦品种成熟胚愈伤诱导与分化研究 | |
| 引用本文： | 李和平,王韬,黄涛,张月芝,李梅芳,廖玉才.不同大麦品种成熟胚愈伤诱导与分化研究[J].华中农业大学学报,2007,26(5):599-602. |
| 作者姓名： | 李和平 王韬 黄涛 张月芝 李梅芳 廖玉才 |
| 作者单位： | 1. 华中农业大学生命科学技术学院,武汉,430070 2. 湖北省农业科学院作物研究所,武汉,430068 3. 华中农业大学植物科学技术学院,武汉,430070 |
| 基金项目： | 国家自然科学基金项目(30470929,30530510)，教育部博士点基金资助 |
| 摘 要： | 为了筛选不受生长季节限制的大麦遗传转化受体,分析、比较了我国15个大麦品种成熟胚在1种诱导培养基和3种分化培养基上的愈伤诱导和分化率。结果表明,大麦品种间成熟胚愈伤诱导率差异非常显著,早熟3号的诱导率高达98.7%,而扬农啤2号和鄂大麦9号则低于10.0%。成熟胚在形成愈伤组织过程中,伴随胚发芽现象,但愈伤诱导与胚发芽之间没有相关性;品种间愈伤组织分化率也差异显著,早熟3号的分化率为43.3%,而其它品种的分化率均在10.0%以下;不同分化培养基的分化效率不同,在M1培养基上的平均分化率较高。 |
| 关 键 词： | 大麦 愈伤诱导 分化 成熟胚 |
| 文章编号： | 1000-2421（2007）05-0599-04 |
| 修稿时间： | 2007-03-25 |
Callus Induction and Differentiation from Mature Embryos of Different Elite Barley Varieties |
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| LI He-ping,WANG Tao,HUANG Tao,ZHANG Yue-zhi,LI Mei-fang,LIAO Yu-cai.Callus Induction and Differentiation from Mature Embryos of Different Elite Barley Varieties[J].Journal of Huazhong Agricultural University,2007,26(5):599-602. | |
| Authors: | LI He-ping WANG Tao HUANG Tao ZHANG Yue-zhi LI Mei-fang LIAO Yu-cai |
| Institution: | 1. College of Life Science and Technology, Huazhong Agricultural University ,Wuhan 430070, China; 2. Institute of Crops, Hubei Academy of Agricultural Sciences , Wuhan 430068, China; 3. College of Plant Science and Technology, Huazhong Agricultural University ,Wuhan 430070,China |
| Abstract: | To screen barley varieties suitable for genetic transformation that is not constrained by seasonal restrictions in field,fifteen elite barley varieties from China were compared for their callus induction and differentiation derived from mature embryos on different media.The results indicated that there was a significant difference of callus induction frequencies in different varieties.Zaoshu 3 displayed a frequency of 98.7%,whereas Yangnongpi 2 and Edamai 9 had a frequency of less than 10.0% under the same conditions.Although mature embryos germinated during callus induction no significantly correlation was observed between germination and callus induction.Furthermore,differentiation frequencies among different varieties also varied significantly.Zaoshu 3 had a frequency of 43.3% and the frequencies for all the remaining varieties were less than 10.0%.Three differentiation media had different effects on differentiation of barley varieties,and M1 medium was better than M2 and M3. |
| Keywords: | barley callus induction differentiation mature embryo |
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