| 转反义trxs基因小麦种子萌发过程中碳代谢的变化 |
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| 引用本文： | 任江萍,李磊,王新国,王振云,尹钧.转反义trxs基因小麦种子萌发过程中碳代谢的变化[J].中国农学通报,2008,24(11):251-254. |
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| 作者姓名： | 任江萍 李磊 王新国 王振云 尹钧 |
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| 作者单位： | 河南农业大学/国家小麦工程技术研究中心,郑州,450002 |
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| 基金项目： | 河南省杰出青年科学基金 |
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| 摘 要： | 为了揭示反义硫氧还蛋白基因（anti-trxs）在抗穗发芽小麦中的作用机制。以转反义 trxs 基因小麦株系为材料，对转基因株系和对照种子萌发过程中硫氧还蛋白h活性、淀粉酶活性、可溶性糖和淀粉含量进行了检测。结果表明，转基因小麦籽粒 trxh、α-淀粉酶和β-淀粉酶活性均显著低于对照；淀粉降解和可溶性糖生成速率明显下降。发芽1~5 d，转基因小麦种子trxh、α-淀粉酶和β-淀粉酶活性分别比对照下降24.5%、40.4%和23.0%；淀粉降解和可溶性糖生成速率分别比对照降低23.7%和23%。
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| 关 键 词： | 农村贫困地区 农村贫困地区 良性耦合 补偿机制 |
| 收稿时间： | 2008-09-04 |
| 修稿时间： | 2008-09-18 |
Change of carbon metabolism in Transgenic Anti-sense Thioredoxin s Gene Wheat in the process of germination |
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| Ren Jiangping,Li Lei,Wang Xinguo,Wang Zhenyun,Yin Jun.Change of carbon metabolism in Transgenic Anti-sense Thioredoxin s Gene Wheat in the process of germination[J].Chinese Agricultural Science Bulletin,2008,24(11):251-254. |
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| Authors: | Ren Jiangping Li Lei Wang Xinguo Wang Zhenyun Yin Jun |
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| Abstract: | To clarify the function mechanism of antisense - thioredoxin s (anti-trxs) gene in transgenic wheat with pre-harvest resistant sprouting characteristic, the thioredoxin h and amylase activities, starch and soluble sugar contents in transgenic line and non-transgenic cultivar ‘Yumai 34’ were detected during seed germination stage. The results indicated that the activities of thioredoxin h, α- amylase and β-amylase were lowered significantly and the velocities of starch degradation and soluble sugar production decreased distinctly. During germinating days 1-5d, the activities of thioredoxin h, α- amylase and β-amylase were 24.5%, 40.4% and 23.1% lower than non-transgenic seeds, respectively. The velocities of starch degradation and soluble sugar production decreased by 23.7% and 23%, respectively. |
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| Keywords: | wheat anti-sense trxs gene trxh activity carbon metabolism |
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