首页 | 本学科首页   官方微博 | 高级检索  
     检索      

产毒素多杀性巴氏杆菌的鉴定
引用本文:李伟杰,赵耘,杜昕波,康凯,陈敏.产毒素多杀性巴氏杆菌的鉴定[J].中国畜牧兽医,2010,37(1):149-151.
作者姓名:李伟杰  赵耘  杜昕波  康凯  陈敏
作者单位:(中国兽医药品监察所, 北京 100081)
基金项目:国家科技支撑计划(2006BAD06A11)
摘    要:采用菌落多重PCR方法对分离保存的28株多杀性巴氏杆菌进行种型和毒素基因的检测,结果表明,菌株C51-6、M-4和P-2237为产毒素多杀性巴氏杆菌,菌株C51-6和P-2237为荚膜血清D型,菌株M-4为荚膜血清A型。同时用金黄色葡萄球菌抑制试验、中性吖啶黄沉淀试验和豚鼠皮肤坏死试验对PCR方法进行了验证。基于对甘露醇、卫茅醇、山梨醇、海藻糖的发酵能力和产生鸟氨酸脱羧酶的特性,3株菌株鉴定为多杀性巴氏杆菌多杀亚种。

关 键 词:产毒素多杀性巴氏杆菌  多重PCR  皮肤坏死毒素  

Identification of Toxigenic Pasteurella multocida
LI Wei-jie,ZHAO Yun,DU Xin-bo,KANG Kai,CHEN Min.Identification of Toxigenic Pasteurella multocida[J].China Animal Husbandry & Veterinary Medicine,2010,37(1):149-151.
Authors:LI Wei-jie  ZHAO Yun  DU Xin-bo  KANG Kai  CHEN Min
Institution:(China Institute of Veterinary Drug Control, Beijing 100081, China)
Abstract:Colony multiplex PCR assays for species,capsular type and dermonecrotic toxin were conducted to identify twenty-eight Pasteurella multocida strains conserved.The results showed that strains C51-6,M-4 and P-2237 were toxigenic Pasteurella multocida,strains C51-6 and P-2237 were serotype D and strain M-4 belonged to serotype A.At the same time,Staphylococcal inhibitory test,neutral acriflavine precipitation and guinea pig skin test were conducted to verify the PCR assays.Based on the ability to utilize mannit...
Keywords:toxigenic Pasteurella multocida  multiplex PCR  dermonecrotic toxin  
本文献已被 CNKI 维普 万方数据 等数据库收录!
点击此处可从《中国畜牧兽医》浏览原始摘要信息
点击此处可从《中国畜牧兽医》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号