| 拮抗放线菌M18的分离鉴定及杀菌活性研究 |
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| 引用本文： | 马海丹,周文明,吴绍东.拮抗放线菌M18的分离鉴定及杀菌活性研究[J].西北农业学报,2007,16(5):291-294. |
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| 作者姓名： | 马海丹 周文明 吴绍东 |
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| 作者单位： | 西北农林科技大学理学院,陕西杨凌,712100 |
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| 摘 要： | 从山西大同地区采集的土样中分离到一株具有广谱杀菌活性的链霉菌菌株M18,经初步鉴定为链霉菌属的黄色类群(Streptomyces. flavus)。杀菌活性测定表明:M18菌株与大肠杆菌、金黄色葡萄球菌、烟草赤星病菌、马铃薯干腐病菌有拮抗作用,抑菌圈透明且直径达到了20 mm以上。M18菌株的发酵原液对西瓜枯萎病菌、番茄早疫病菌、苹果轮纹病菌、玉米大斑病菌、小麦赤霉病菌、苹果炭疽病菌的菌丝生长抑制率均在75%以上,对苹果腐烂病菌、玉米大斑病菌、棉花枯萎病菌孢子的萌发抑制率在50%以上,对假丝酵母菌、枯草芽孢杆菌有明显抑制的作用。
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| 关 键 词： | 链霉菌 筛选 杀菌活性 生物测定 |
| 文章编号： | 1004-1389（2007）05-0291-04 |
| 收稿时间： | 2007-01-12 |
| 修稿时间： | 2007-04-15 |
Studies on Isolation, Identification and Fungicidal Activity of Antagonistic Actinomycete M18 |
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| MA Hai-dan,ZHOU Wen-ming and WU Shao-dong.Studies on Isolation, Identification and Fungicidal Activity of Antagonistic Actinomycete M18[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(5):291-294. |
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| Authors: | MA Hai-dan ZHOU Wen-ming and WU Shao-dong |
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| Institution: | College of Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;College of Sciences, Northwest A & F University, Yangling Shaanxi 712100, China;College of Sciences, Northwest A & F University, Yangling Shaanxi 712100, China |
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| Abstract: | A actinomycete strain producing a broad-spectrum antifungal substance was isolated from the soil in Datong Shanxi.The strain numbered M18 was initially classified into Streptomyces.flavus.The result indicated that M18 has antagonism against Escherichia coli,Staphylococcus aureus,Fusarium solani and Alernaria longipes,the diameter of inhibition zone were over 20 mm.Studied by mycelium growth method,result showed that the metabolite had an inhibition rate of mycelium growth over 75% against six kinds of fungi.Researched by spores germination method,the authors found that it also had an inhibition rate above 50% against germination of spores of there kinds of fungi.And it could inhibit the growth of two kinds of bacteria. |
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| Keywords: | Streptomyces Screening Fungicidal activity Bioassay Identified |
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