| 抑制剂和激活剂对梨星毛虫中肠蛋白酶活性的影响 |
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| 引用本文： | 董艳璐,张战利,吴江波,陈 玮,袁向群,李怡萍.抑制剂和激活剂对梨星毛虫中肠蛋白酶活性的影响[J].西北农业学报,2022(9):1235-1242. |
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| 作者姓名： | 董艳璐 张战利 吴江波 陈 玮 袁向群 李怡萍 |
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| 作者单位： | (1.西北农林科技大学 植物保护学院，农业部西北黄土高原作物有害生物综合治理重点实验室，陕西杨凌 712100；2.陕西省植物保护工作总站，西安 710003；3.宜川县农业技术推广服务中心，陕西宜川 716200) |
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| 基金项目： | 国家自然科学基金（31871971，32172409）；陕西省科技重大专项（2020ZDZX03-03-02）。 |
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| 摘 要： | 旨在研究不同抑制剂和激活剂对梨星毛虫Illiberis pruni中肠蛋白酶活性的影响。利用生化技术分别测定梨星毛虫幼虫不同龄期的4种中肠蛋白酶（总蛋白酶、强碱性胰蛋白酶、弱碱性胰蛋白酶和胰凝乳蛋白酶）活性，进而在最适龄期（4龄幼虫）测定蛋白酶激活剂（EDTA，MgCl2，EGTA，CaCl2）和抑制剂（PMSF，TPCK，STI，TLCK，IAA，DTT）对梨星毛虫中肠蛋白酶活性的影响。结果表明：梨星毛虫幼虫中肠总蛋白酶、强碱性胰蛋白酶、弱碱性胰蛋白酶、胰凝乳蛋白酶均在4龄具有最高酶活性。所有试剂均对总蛋白酶有激活作用；5 mmol/L CaCl2对强碱性胰蛋白酶抑制作用最高；对弱碱性胰蛋白酶有抑制作用的仅有5 mmol/L DTT；对胰凝乳蛋白酶抑制效果最佳的为10 mmol/L EGTA。所筛选的对梨星毛虫主要中肠蛋白酶具有抑制作用的抑制剂，对以昆虫中肠蛋白酶为靶标的绿色害虫防控技术的开发具有参考价值。
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| 关 键 词： | 梨星毛虫 中肠 蛋白酶 活性 抑制剂 |
Effects of Inhibitors and Activators on Protease Activity in the Midgut of Illiberis pruni |
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| DONG Yanlu,ZHANG Zhanli,WU Jiangbo,CHEN Wei,YUAN Xiangqun and LI Yiping.Effects of Inhibitors and Activators on Protease Activity in the Midgut of Illiberis pruni[J].Acta Agriculturae Boreali-occidentalis Sinica,2022(9):1235-1242. |
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| Authors: | DONG Yanlu ZHANG Zhanli WU Jiangbo CHEN Wei YUAN Xiangqun and LI Yiping |
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| Abstract: | This paper aims to investigate the effects of different inhibitors and activators on the midgut protease activity of the Illiberis pruni.Determination of the activities of four midgut proteases (total protease, high-alkaline trypsin, low-alkaline trypsin and chymotrypsin) in different instar larvae of Illiberis pruni by biochemical techniques, and then the effects of protease activators (EDTA, MgCl2, EGTA, MgCl2) and inhibitors (PMSF, TPCK, STI, TLCK, IAA, DTT) on the midgut protease activity of Illiberis pruni were determined at the optimum age (4th instar larvae).The results showed that total protease, high-alkaline trypsin, low-alkaline trypsin and chymotrypsin have the highest enzymatic activities at the 4th instar.Under the optimum conditions, all reagents have activating effects on total protease; 5 mmol/L CaCl2 have the highest inhibitory effect on high-alkaline trypsin; only 5 mmol/L DTT have inhibitory effect on low-alkaline trypsin; 10 mmol/L EGTA have the best inhibitory effect on chymotrypsin.The screened inhibitor has inhibitory effect on the main midgut proteases of Illiberis pruni, which has reference value for the development of green pest control technology targeting insect midgut proteases. |
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| Keywords: | Illiberis pruni Midgut Proteases Active Inhibitors |
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