Novel application of square-wave adsorptive-stripping voltammetry for the determination of xanthohumol in spent hops |
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| Authors: | Moreira Manuela M Carvalho Ana M Valente Ines M Goncalves Luis M Rodrigues Jose A Barros Aquiles A Guido Luis F |
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| Institution: | Departamento de Qui?mica e Bioqui?mica, Universidade do Porto, Porto, Portugal. |
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| Abstract: | This paper reports the development of a novel electrochemical assay for xanthohumol (XN) by square-wave adsorptive-stripping voltammetry (SWAdSV) with a hanging mercury drop electrode. The method showed good repeatability (CV < 2%) and linearity (between 10 and 250 μg L(-1)), as well as suitable limits of detection (2.6 μg L(-1)) and quantification (8.8 μg L(-1)). The method was applied for the quantification of this compound in spent hops, and the results obtained were compared with the HPLC-UV method. XN contents determined by the SWAdSV method were 16 ± 1 and 100 ± 4 μg L(-1) for aqueous and methanolic extracts, respectively. The developed new methodology considerably reduces the analysis time, approximately from 25 min (HPLC-UV method) to 7 min, enabling a high sample throughput. In addition, the detection and quantification limits were approximately 5-fold lower than those obtained with the chromatographic method. |
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