| 胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价 |
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| 引用本文： | 张凯,吴曼莉,辜柳霜,郑钧月,柳志强,李晓宇.胶孢炭疽菌生防放线菌gz-8的鉴定及生物活性初步评价[J].农药学学报,2017,19(6):708-715. |
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| 作者姓名： | 张凯 吴曼莉 辜柳霜 郑钧月 柳志强 李晓宇 |
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| 作者单位： | 1.海南大学 热带农林学院, 海口 570228 |
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| 基金项目： | 海南省重点研发计划（ZDYF2016155）；国家自然科学基金（31560045） |
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| 摘 要： | 从山东省临沂地区土壤中分离到一株放线菌gz-8，发现其对胶孢炭疽菌Colletotrichum gloeosporioides具有较强的抑制作用，皿内抑菌活性达72.5%。通过形态特征和生理生化测定，结合16S rDNA序列分析，鉴定该菌株为栗褐链霉菌Streptomyces badius。抗菌谱测定结果表明，菌株gz-8对6种植物病原真菌均具有较强的抑菌活性，抗菌谱较广。进一步对菌株gz-8的粗提物进行了生物活性评价，发现其发酵液粗提物可以明显抑制胶孢炭疽菌的菌丝生长（EC50=3.18 μg/mL）和分生孢子萌发（IC50=1.92 μg/mL），且活性明显高于菌体粗提物；同时发酵液粗提物具有良好的热稳定性及pH稳定性；1 000 mg/L的发酵液粗提物对感染胶孢炭疽病芒果果实的防效达到35.94%，与同浓度下百菌清的防效（34.11%）无显著差异。
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| 关 键 词： | 胶孢炭疽菌 放线菌 栗褐链霉菌 炭疽病 菌株鉴定 生物防治 |
| 收稿时间： | 2017/6/27 0:00:00 |
Identification and preliminary evaluation of a biocontrol actinomycete strain gz-8 against Colletotrichum gloeosporioides |
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| ZHANG Kai,WU Manli,GU Liushuang,ZHENG Junyue,LIU Zhiqiang and LI Xiaoyu.Identification and preliminary evaluation of a biocontrol actinomycete strain gz-8 against Colletotrichum gloeosporioides[J].Chinese Journal of Pesticide Science,2017,19(6):708-715. |
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| Authors: | ZHANG Kai WU Manli GU Liushuang ZHENG Junyue LIU Zhiqiang and LI Xiaoyu |
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| Institution: | 1.Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China |
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| Abstract: | An actinomycete strain gz-8 was isolated from Linyi, Shandong Province, which had a strong antagonistic effect against Colletotrichum gloeosporioides with the inhibition rate of 72.5%. Strain gz-8 was identified as Streptomyces badius based on the morphology, physiological and biochemical characteristics, combined with 16S rDNA sequence analysis. Strain gz-8 has a broad antifungal spectrum, with strong inhibitory effects on six plant pathogenic fungi. The inhibitory activity of the crude extract from strain gz-8 was evaluated. The fermentation broth extract exhibits obvious inhibitory effects on the mycelial growth (EC50=3.18 μg/mL) and conidial germination (IC50=1.92 μg/mL) of C. gloeosporioides, which had higher activity compared to the mycelium extract of strain gz-8. The fermentation broth extract had good thermostability and pH stability. The control effect of 1 000 mg/L fermentation broth extract to mango fruits infected with C. gloeosporioides was 35.94%, which had no significant difference with that of chlorothalonil at the same concentration(34.11%). |
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| Keywords: | Colletotrichum gloeosporioides anthracnose actinomycete strain Streptomyces badius strain identification biological control |
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