| 高效液相色谱法测定盐酸多西环素可溶性粉的含量 |
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| 引用本文： | 李军.高效液相色谱法测定盐酸多西环素可溶性粉的含量[J].中国兽药杂志,2009,43(7):9-12. |
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| 作者姓名： | 李军 |
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| 作者单位： | 广西兽药监察所 |
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| 摘 要： | 采用高效液相色谱法（HPLC）测定盐酸多西环素可溶性粉中多西环素的含量,并对检验方法1]进行复核验证。色谱柱W aters Nova.pakR○C18（3.9 mm×150 mm,4μm）;以0.05 mol/L草酸铵溶液-二甲基甲酰胺-0.2 mol/L磷酸氢二铵溶液（65∶30∶5）（用氨试液调节pH值为8.0）为流动相;柱温35℃;检测波长280 nm;流速1.0 mL/min;进样量20μL。多西环素进样浓度在4.0-144.0μg/mL的范围内与峰面积呈良好的线性关系（r=1.000）;样品的平均加样回收率（n=9）为98.5%,RSD为2.0%;完成了185批次样品的检验,检验结果准确可靠。该方法准确度高,重复性好,结果可靠,能够有效地检测盐酸多西环素可溶性粉的含量。
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| 关 键 词： | 高效液相色谱法 盐酸多西环素可溶性粉 测定 |
| 收稿时间： | 2009/2/27 0:00:00 |
| 修稿时间： | 2009/4/20 0:00:00 |
Determination of Doxycycline Hyclate Powder |
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| li jun.Determination of Doxycycline Hyclate Powder[J].Chinese Journal of Veterinary Drug,2009,43(7):9-12. |
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| Authors: | li jun |
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| Institution: | Guangxi Veterinary medicine Inspection Institute |
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| Abstract: | The contents of doxycycline in doxycycline hyclate powder were detected by high performance liquid chromatography and the determination was re -validated. Column Waters Nova. pal C18 （3.9 mm × 150 mm,4 μm） with 0.05 mol/L diammonium oxalate -N, N- dimethylfromamide -0.2 mol/mL diammonium dydrogen phosphate （65 ： 30 ： 5 ） （ pH 8.0 regulated with diammonium） was used as mobile phase. The column temperature was 35 ℃. The detection wavelength was 280 nm. The flow rate was 1.0 mL/min. The injection volume was 20 μL. There was a good linear correlation between the peak areas and the concentrations of doxycycline and the range from 4.0 - 144.0 μg/mL（ r = 1. 000）. The average recovery was 98.5% and RSD was 2.0% （ n = 9）. 185 samples had been detected and the results were accurate and reliable. The results indicated that the constructed method was accurate, repeatable and reliable. |
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| Keywords: | HPLC doxycycline hyclate powder determination |
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