| 四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究 |
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| 引用本文： | 涂蕊,但佳明,严光文,郑静,田一男,肖启程,魏斌,周紫峣,刘海峰,杨亭玉,彭广能,钟志军.四川省部分地区成年山羊十二指肠贾第虫感染调查与基因分型研究[J].华南农业大学学报,2018,39(5):59-64. |
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| 作者姓名： | 涂蕊 但佳明 严光文 郑静 田一男 肖启程 魏斌 周紫峣 刘海峰 杨亭玉 彭广能 钟志军 |
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| 作者单位： | 四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室;西昌学院动物科技学院;南充市顺庆区农牧业局 |
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| 基金项目： | 国家重点研发计划（2016YFD0501009）；国家自然科学基金（31000548）；成都大熊猫繁育研究基金（CPF2015-4，CPF2017-05）；四川农业大学本科生科研兴趣培养项目（ky2016076） |
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| 摘 要： | 【目的】了解四川省部分地区成年山羊贾第虫Giardia duodenalis感染情况以及感染虫种基因型。【方法】在四川省12个养殖场采集了342份成年山羊的新鲜粪便,采用多位点基因分型技术,通过巢式PCR扩增bg、tpi和gdh基因位点,对阳性样品的PCR产物进行了测序分析。【结果】342份成年山羊粪样中贾第虫的总感染率为14.91%(51/342)。所有阳性样品感染的虫种基因型均为集聚体E。51份阳性样品在bg、tpi和gdh这3个基因位点分别成功鉴定出4(E5、E8、E17、E18)、2(E2、E4)和2(E3、E4)种基因亚型,并且在bg位点发现了2种新的基因亚型(E17、E18)。【结论】四川省部分地区成年山羊存在较为严重的贾第虫感染,虫种类型主要为集聚体E,且具有一定的遗传多样性。
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| 关 键 词： | 成年山羊 贾第虫 多位点基因分型技术 集聚体E |
| 收稿时间： | 2018/1/28 0:00:00 |
Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas |
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| TU Rui,DAN Jiaming,YAN Guangwen,ZHENG Jing,TIAN Yinan,XIAO Qicheng,WEI Bin,ZHOU Ziyao,LIU Haifeng,YANG Tingyu,PENG Guangneng and ZHONG Zhijun.Prevalence and genotyping of Giardia duodenalis in adult goats in Sichuan partial areas[J].Journal of South China Agricultural University,2018,39(5):59-64. |
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| Authors: | TU Rui DAN Jiaming YAN Guangwen ZHENG Jing TIAN Yinan XIAO Qicheng WEI Bin ZHOU Ziyao LIU Haifeng YANG Tingyu PENG Guangneng and ZHONG Zhijun |
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| Institution: | College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Animal Science and Technology, Xichang College, Xichang 615000, China,Farming and Animal Husbandry Bureau in Shunqing District of Nanchong City, Nanchong 637000, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China,College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China and College of Veterinary Medicine, Sichuan Agricultural University/Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China |
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| Abstract: | |
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| Keywords: | adult goat Giardia duodenalis multilocus genotyping technique assemble E |
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