| 砂梨上苹果茎痘病毒的调查分析及RT-PCR与TC-RT-PCR检测研究 |
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| 引用本文： | 侯雨萱,洪霓,邓晓云,胡红菊,王国平.砂梨上苹果茎痘病毒的调查分析及RT-PCR与TC-RT-PCR检测研究[J].果树学报,2005,22(4):343-346. |
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| 作者姓名： | 侯雨萱 洪霓 邓晓云 胡红菊 王国平 |
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| 作者单位： | 1. 华中农业大学植物科技学院,武汉,430070
2. 湖北省农业科学院果茶蚕桑研究所,武汉,430209 |
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| 基金项目： | 科技部863课题(项目编号:2001AA241142)。 |
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| 摘 要： | 试验通过西方烟(Nicotianaoccidentalis‘37B’)和杂种(Pyroniaveitchii)、A20接种鉴定及PAS-ELISA检测,对保存于国家砂梨种质资源圃的38份砂梨样品进行了苹果茎痘病毒(Applestempittingvirus,ASPV)带毒率的调查分折。结果表明,生物学鉴定与血清学检测结果一致,ASPV带毒率均为26.3%。应用以dsRNA为模板RT-PCR检测该病毒,在杂种和A20上显症的6个样品均获得316bp目标片段。应用TC-RT-PCR检测显症的西方烟,也获得了预期的目标片段。
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| 关 键 词： | 苹果茎痘病毒 生物学鉴定 PAS-ELISA dsRNA RT-PCR TC-RT-PCR |
| 文章编号： | 1009-9980(2005)04-343-04 |
| 修稿时间： | 2004年10月22 |
Investigation and detection of Apple stem pitting virus in Pyrus pyrifolia by RT-PCR and TC-RT-PCR |
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| HOU Yu-xuan,Hong Ni,DENG Xiao-yun,HU Hong-ju,WANG Guo-ping.Investigation and detection of Apple stem pitting virus in Pyrus pyrifolia by RT-PCR and TC-RT-PCR[J].Journal of Fruit Science,2005,22(4):343-346. |
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| Authors: | HOU Yu-xuan Hong Ni DENG Xiao-yun HU Hong-ju WANG Guo-ping |
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| Institution: | HOU Yu-xuan1,HONG Ni1,DENG Xiao-yun1,HU Hong-ju2,WANG Guo-ping1* |
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| Abstract: | In this study,38 samples of pear cultivars belonging to Pyrus pyrifolia were collected in the National Fruit Germplasm Repository of Pyrus pyrifolia for testing and detecting the Apple stem pitting virus (ASPV) by Nicotiana occidentalis '37B',Pyronia veitchii and A20 inoculated and PAS-ELISA. Results showed the detective consequence by PAS-ELISA was consistent with that by biological index, the detection rate reached up to 26.3%. RT-PCR using dsRNA as template was performed to detect ASPV and the expected fragment about 316 bp was amplified in 6 samples on Pyronia veitchii Aspv-affected. The corresponding fragment was also amplified by TC-RT-PCR on Nicotiana occidentalis '37B' Aspv-affected. |
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| Keywords: | ASPV Biological index PAS-ELISA dsRNA RT-PCR TC-RT-PCR |
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