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| 腐烂茎线虫rDNA-ITS序列分析 | |
| 引用本文： | 刘先宝,谭志琼,葛建军.腐烂茎线虫rDNA-ITS序列分析[J].热带作物学报,2008,29(3):385-389. |
| 作者姓名： | 刘先宝 谭志琼 葛建军 |
| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南省热带农业有害生物检测监控重点实验室,海南,儋州,571737 2. 海南大学环境与植物保护学院,海南,儋州,571737 3. 中国检验检疫科学院动植物检疫研究所,北京,100029 |
| 基金项目： | 国家科技支撑计划-进境有害生物检测关键技术研究项目 |
| 摘 要： | 利用一对通用引物rDNA1/rDNA2扩增6个腐烂茎线虫供试群体的rDNA-ITS区域,获得序列长度不等的片段.Des-1、Des-2和Des-3群体扩增片断长度均为1 130 bp.而Des-5、Des-6和Des-7群体的扩增片段长度均为942 bp,与以上群体相比,在ITS区具有一个188 bp的缺失片段.序列比对后确定缺失片段位于ITS1区,而且该片段含有多个可重复小片段,这可能是造成碱基缺失的原因.根据遗传进化分析可将供试线虫分为两个大的群体. |
| 关 键 词： | 腐烂茎线虫 rDNA-ITS 序列分析 |
Sequence Analysis of Internal Transcribed Spacer of Ditylenchus destructor Ribosomal DNA |
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| Liu Xianbao,Tan Zhiqiong and Ge Jianjun.Sequence Analysis of Internal Transcribed Spacer of Ditylenchus destructor Ribosomal DNA[J].Chinese Journal of Tropical Crops,2008,29(3):385-389. | |
| Authors: | Liu Xianbao Tan Zhiqiong and Ge Jianjun |
| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Key Laboratory for Pest Detection and Monitoring of Tropical Agriculture;College of Environment and Plant Protection, Hainan University;Institute of Animal and Plant Quarantine, Chinese Academy of Quarantine and Inspection |
| Abstract: | The internal transcribed spacer(ITS) of rDNA from 6 nematode(Ditylenchus destructor) populations in 3 host plants were amplified and sequenced. The amplification of the rDNA-ITS region from Des-1, Des-2 and Des-3 populations produced one single fragment of 1 130 bp, whereas the PCR products of the ITS from Des-5, Des-6 and Des-7 populations were 942 bp. The ITS section was proved by the sequence of rDNA-ITS region from the GenBank. There was an absent fragment of 188 bp in the ITS1 section of Des-5, Des-6 and Des-7 populations. In the absent section were found some small repeated fragments which might be the main reason for the absence in the fragment. The 6 nematodes can be grouped into two large populations based on the analysis of their genetic evolution. |
| Keywords: | Ditylenchus destructor rDNA-ITS sequence analysis |
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