| 嵌套式PCR扩增SRY基因序列鉴定牛胚胎性别体系的建立 |
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| 引用本文： | 白文林,尹荣焕,孙明亮,周晨阳,纪英卓,罗光彬.嵌套式PCR扩增SRY基因序列鉴定牛胚胎性别体系的建立[J].湖北农业科学,2006,45(4):406-409. |
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| 作者姓名： | 白文林 尹荣焕 孙明亮 周晨阳 纪英卓 罗光彬 |
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| 作者单位： | 1. 沈阳农业大学畜牧兽医学院,沈阳,110161
2. 辽宁省家畜家禽遗传资源保存利用中心,辽宁,辽阳,111000 |
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| 基金项目： | 辽宁省教育厅资助项目
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沈阳农业大学校科研和教改项目 |
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| 摘 要： | 应用源自牛X和Y染色体同源区的1对外引物P1、P2及其各自特异区的2对内引物P3、P4和P5、P6,进行嵌套式PCR反应,对中国荷斯坦公、母牛静脉血样DNA及牛胚胎样DNA进行特异性条带的嵌套式PCR扩增,并对外、内嵌套式引物的PCR反应体系进行了优化,以准确鉴定牛早期胚胎性别.结果表明,在优化了的PCR反应体系下,中国荷斯坦公牛DNA样品得到178bp和262bp的两个条带,而母牛仅得到262bp的1个条带;静脉血样DNA性别鉴定结果与实际性别相符率为100%,表明本试验建立的体系完全可以用于牛早期的胚胎性别鉴定.
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| 关 键 词： | 牛 早期胚胎 嵌套式聚合酶链式反应分子标记 SRY基因 性别鉴定 |
| 文章编号： | 0439-8114(2006)04-0406-03 |
| 收稿时间： | 04 28 2006 12:00AM |
| 修稿时间： | 2006年4月28日 |
Construction of Sex Determination System of Bovine Embryos by Nested PCR Amplifying the Sequence of SRY Gene |
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| BAI Wen-lin,YIN Rong-huan,SUN Ming-liang,ZHOU Chen-yang,JI Ying-zhuo,LUO Guang-bin.Construction of Sex Determination System of Bovine Embryos by Nested PCR Amplifying the Sequence of SRY Gene[J].Hubei Agricultural Sciences,2006,45(4):406-409. |
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| Authors: | BAI Wen-lin YIN Rong-huan SUN Ming-liang ZHOU Chen-yang JI Ying-zhuo LUO Guang-bin |
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| Institution: | 1.College of Anhnal Science and Veterinary,Shenyang Agrieuhural University, Shenyang 110161, China; 2.Conservation and Utilization Center of Domestic Animal and Poultry Genetic Resources in Liaoning Province, Liaoyang 111000. Liaoning, China |
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| Abstract: | The sex specific band was identified from bull,cow blood sample and preimplantation embryos DNA of China Holsteins by outer primers P1,P2 from complementary region of X chromosome and Y chromosome,inner primers P3,P4 and P5,P6 from specific region of X chromosome and Y chromosome,and the system of PCR reaction of outer and inner primers was optimized so as to determine precisely the sex of early embryo.The result showed that under the system optimized,bull demonstrated two bands of 178bp and 262bp,and cow only demonstrated a band of 262bp.The result of blood DNA identification was completely in accordance with the actual sex type.Therefore,the system constructed in this experiment could be used completely to determine the sex of bovine early embryo. |
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| Keywords: | Bovine early embryo nested PCR SRY gene sex determination |
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