| 扶桑绵粉蚧钙调蛋白基因的克隆与生物信息学分析 |
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| 引用本文： | 罗梅,董章勇,宾淑英,林进添,吴仲真,李献锋.扶桑绵粉蚧钙调蛋白基因的克隆与生物信息学分析[J].华中农业大学学报,2012,31(3):320-324. |
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| 作者姓名： | 罗梅 董章勇 宾淑英 林进添 吴仲真 李献锋 |
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| 作者单位： | 仲恺农业工程学院植物保护系/外来有害生物预警与控制研究所,广州,510225 |
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| 基金项目： | 农业部公益性行业科研专项（201103026）和仲恺农业工程学院科研基金项目（G3100004） |
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| 摘 要： | 钙调蛋白(calmodulin,CaM)对生物体内多种Ca2+依赖的细胞功能和酶体系都有重要的调节作用。为研究扶桑绵粉蚧的信号转导受体蛋白,首次克隆了扶桑绵粉蚧钙调蛋白基因PsCaM的cDNA全长序列,其开放阅读框(ORF)包含447bp的片段,编码148个氨基酸。PsCaM基因由3个内含子和4个外显子组成。3个内含子的长度分别为73、81、72bp,分隔的4个外显子的长度分别为33、133、183、98bp。功能域分析结果显示:该蛋白具有2个EF-hand结构域,有13个Ca2+结合位点;该蛋白的理论等电点是6.21,属于稳定蛋白,且没有跨膜区域;通过同源建模获得了其蛋白的三维结构。多序列比较显示,PsCaM基因相对较保守。
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| 关 键 词： | 扶桑绵粉蚧 钙调蛋白 基因 克隆 生物信息学 |
| 收稿时间： | 2011/11/12 0:00:00 |
Molecular cloning and bioinformatics analysis of calmodulin genes in Phenacoccus solenopsis Tinsley |
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| LUO Mei
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DONG Zhang-yong
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BIN Shu-ying
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LIN Jin-tian
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WU Zhong-zhen
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LI Xian-feng.Molecular cloning and bioinformatics analysis of calmodulin genes in Phenacoccus solenopsis Tinsley[J].Journal of Huazhong Agricultural University,2012,31(3):320-324. |
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| Authors: | LUO Mei DONG Zhang-yong BIN Shu-ying LIN Jin-tian WU Zhong-zhen LI Xian-feng |
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| Institution: | Department of Plant Protection,Zhongkai University of Agriculture and Engineering/Institute for Management of Invasive Alien Species,Guangzhou 510225,China |
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| Abstract: | Calmodulin(CaM) plays an important regulatory role in a variety of in vivo Ca2+-dependent cell functions and enzyme systems.It was the first time to clone the calmodulin gene from Phenacoccus solenopsis Tinsley.The full-length of open reading frame(ORF) is 447 bp,encoding 148 amino acid residues.PsCaM gene was constituted by three introns and four exons.The intron lengths were 73,81,72 bp;The lengths of the 4 separated exons were 33,133,183,98 bp.Functional domain analysis showed that the protein got two EF-hand domains,with 13 Ca2+ binding sites.The theoretical protein isoelectric point is 6.21.It was stable protein,with no transmembrane region.The three-dimensional structure of its protein was obtained by homology modeling.Multiple sequence comparison revealed that the gene is relatively conservative.The research was the basis for further study of the functional mechanisms of calmodulin genes. |
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| Keywords: | Phenacoccus solenopsis Tinsley calmodulin gene clone bioinformatic |
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