| 麦穗鱼的组型分析和DNA含量测定 |
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| 引用本文： | 杨坤,王子健,祝东梅,方礼豹,王卫民.麦穗鱼的组型分析和DNA含量测定[J].华中农业大学学报,2012,31(3):371-375. |
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| 作者姓名： | 杨坤 王子健 祝东梅 方礼豹 王卫民 |
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| 作者单位： | 1. 华中农业大学水产学院,武汉,430070
2. 中国科学院生态环境研究中心,北京,100085 |
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| 基金项目： | 国家水体污染控制与治理科技重大专项（水专项）中的“水环境监测的新技术、新方法研发与应用示范”课题（2009ZX07527-005） |
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| 摘 要： | 采用PHA和秋水仙素活体注射、肾细胞短期培养和空气干燥制片法,对麦穗鱼染色体数目和核型进行研究;以麦穗鱼外周血细胞为样本,鸡血细胞DNA为标准(2.30pg),使用美国贝克曼库尔特公司Cell LabQuanta SC流式细胞仪测定了麦穗鱼二倍体细胞的DNA含量。结果显示:麦穗鱼染色体数目2n=50,未观察到次缢痕及性染色体,亦未发现有随体,核型公式为18m+22sm+10st,NF=90;麦穗鱼二倍体细胞DNA含量为鸡血对照的1.38倍,绝对含量为3.18pg。
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| 关 键 词： | 麦穗鱼 染色体 核型分析 流式细胞术 DNA含量 |
| 收稿时间： | 3/3/2011 12:00:00 AM |
Karyotypic analysis and cellular DNA contents of Pseudorasbora parva |
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| YANG Kun
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WANG Zi-jian
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ZHU Dong-mei
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FANG Li-bao
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WANG Wei-min.Karyotypic analysis and cellular DNA contents of Pseudorasbora parva[J].Journal of Huazhong Agricultural University,2012,31(3):371-375. |
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| Authors: | YANG Kun WANG Zi-jian ZHU Dong-mei FANG Li-bao WANG Wei-min |
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| Institution: | 1.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China; 2.Research Center for Eco-environmental Sciences, Chinese Academy of Sciences,Beijing 100085,China |
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| Abstract: | The chromosome number and karyotype of Pseudorasbora parva were analyzed using a routine method including intraperitoneal injection of PHA and colchicine,cultivation in vivo,and slides preparation with air-drying.Whilst erythrocyte nuclear DNA content of P.parva was determined by using a flow cytometer(Beckman Coulter Inc.,USA) with serving the chicken erythrocytes(their DNA content is 2.30 pg) as reference cells.Results obtained in this study showed that:the diploid chromosome number of P.parva was 50(2n=50);the karyotype of this species was 2n=18 m+22 sm+10 st,NF=90,while the secondary constriction,heterosome and satellite chromosome were not observed;the diploid cellular DNA contents of P.parva was 3.18 pg,which was 1.38 times to the chicken erythrocytes’. |
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| Keywords: | Pseudorasbora parva chromosome karyotypic analysis flow cytometry DNA content |
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