| 响应面法优化玉米须中芦丁的提取工艺及检测 |
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| 引用本文： | 赵海福,魏彦明,董晓宁,王廷璞,赵强,刘文博.响应面法优化玉米须中芦丁的提取工艺及检测[J].草业科学,2013,30(6):964-972. |
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| 作者姓名： | 赵海福 魏彦明 董晓宁 王廷璞 赵强 刘文博 |
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| 作者单位： | 甘肃农业大学动物医学院,甘肃兰州,730070;天水师范学院生命科学与化学学院,甘肃天水,741001;新疆大学化学化工学院,新疆乌鲁木齐,830046 |
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| 基金项目： | 国家自然科学基金，天水师范学院中青年教师科研资助项目 |
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| 摘 要： |
为探讨玉米（Zea mays）须中芦丁提取工艺和检测条件，本试验选取乙醇体积分数、液料比、回流时间和超声时间4个因素，采用响应面法优化玉米须中芦丁的提取工艺，并将最佳工艺条件提取的芦丁样品进行紫外光谱和红外光谱测定。结果表明，玉米须中芦丁的最佳提取工艺条件为乙醇体积分数49%、液料比30 mL∶1 g、回流时间1.7 h以及超声时间36 min。在该工艺条件下，玉米须芦丁含量为12.152 mg·g-1，且经紫外、红外光谱测定，其主要官能团吸收峰显著，HPLC检测重现性好。说明该工艺对玉米须中芦丁的提取优化效果显著，为玉米须深层次研究提供了一定参考。
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| 关 键 词： | 玉米须 芦丁 响应面法 |
Optimum conditions of extracting and detecting rutin from stigma maydis by response surface analysis |
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| ZHAO Hai fu,WEI Yan ming,DONG Xiao ning,WANG Ting pu,ZHAO Qiang,LIU Wen bo.Optimum conditions of extracting and detecting rutin from stigma maydis by response surface analysis[J].Pratacultural Science,2013,30(6):964-972. |
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| Authors: | ZHAO Hai fu WEI Yan ming DONG Xiao ning WANG Ting pu ZHAO Qiang LIU Wen bo |
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| Abstract: | The purpose of this study was to explore optimum conditions of extracting and detecting rutin from stigma maydis. Four factors, including ethanol volumteric concentrations, liquid to solid ratio, reflux time and ultrasonic time were selected to optimize the extraction process of rutin by response surface method. Rutin samples extracted by the optimum process were determined by ultraviolet and infrared spectrometry, and HPLC. The experimental results showed that the best conditions of extracting rutin from stigma maydis were ethanol concentration: 49%, liquid to solid ratio: 30 mL∶1 g, reflux time: 1.7 h and ultrasonic time: 36 min. Under these processing conditions, the rutin content in stigma maydis was 12.152 mg·g-1. The results from ultraviolet and infrared spectrometry indicated that the main functional groups were absorbed significantly. And HPLC detection showed a high repeatability. In conclusion, the optimum conditions selected here on the extraction of rutin from stigma maydis was efficient and provided references to the further study of stigma maydis. |
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| Keywords: | stigma maydis rutin response surface analysis |
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