Monitoring genetic fidelity vs somaclonal variation in Norway spruce (Picea abies) somatic embryogenesis by RAPD analysis |
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| Authors: | Berthold Heinze and Josef Schmidt |
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| Institution: | (1) Biotechnology Department, Austrian Research Centre, A-2444 Seibersdorf, Austria;(2) Present address: Institute of Forest Genetics, Federal Forest Research Centre, Hauptstrasse 7, A-1140 Vienna, Austria |
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| Abstract: | Summary Somaclonal variation, which is a welcome source of genetic variation for crop breeding, is unwanted when direct regenerants have to be used in tissue culture mass propagation (eg. in many forest trees), or in the regeneration of genetically transformed plants. Random amplified polymorphic DNA (RAPD) was used to analyse somatic embryos and plants regenerated from embryogenic cell lines in Norway spruce, Picea abies (L.) Karst. RAPD facilitated the identification of clones, as material from the same cell lines shared identical patterns of amplified fragments, whereas regenerants from different cell lines were easily distinguishable by their respective patterns. For comparisons with explant donor genotypes, cell lines were initiated from cotyledons. Some of the seedlings that had parts of their cotyledons removed were grown on as control plants. Somatic embryos regenerated from cotyledon cell lines showed no aberrations in RAPD banding patterns with respect to donor plants. We conclude that gross somaclonal variation is absent in our plant regeneration system.Abbreviations ESM
embryogenic suspensor mass
- RAPD
random amplified polymorphic DNA
- RFLP
restriction fragment length polymorphism
- (2,4-dichlorophenoxy)acetic acid
2,4-D
- 1-naphthaleneacetic acid
NAA |
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| Keywords: | Picea abies genetic stability somatic embryogenesis RAPD |
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