| 用SDS—PAGE进行鸡败血霉形体结构蛋白分析 |
| |
| 引用本文： | 于圣青,刘晓文,徐步,丁铲.用SDS—PAGE进行鸡败血霉形体结构蛋白分析[J].畜牧兽医学报,2001,32(3):249-252. |
| |
| 作者姓名： | 于圣青 刘晓文 徐步 丁铲 |
| |
| 作者单位： | 江苏省家禽科学研究所, |
| |
| 基金项目： | 江苏省“九·五”重点攻关课题（BE96502） |
| |
| 摘 要： | 利用SDS－PAGE方法对禽败血霉形体R，S6，F株和6株国内分离株进行了结构蛋白比较分析，电泳凝胶染色扫描结果显示，各株之间结构蛋白差异较大，R，S6株P64蛋白表达量较高，D9604株与其相似性较高，D9601，D9603和D9605与F株均有一条特异的75kDa条带，提示我国分离的MG强毒株可能存在着多样性。
|
| 关 键 词： | SDS－PAGE 鸡 败血霉形体 结构蛋白 慢性呼吸道病 粘附机理 |
| 文章编号： | 0366－6964(2001)03－0249－04 |
ANALYSIS OF STRUCTURAL
PROTEINS OF MYCOPLASMA GALLISEPTICUM BY SODIUM DODECYL SULFATE |
| |
| Yu Shengqing,Liu Xiaowen,XU Bu,Ding Chan.ANALYSIS OF STRUCTURAL
PROTEINS OF MYCOPLASMA GALLISEPTICUM BY SODIUM DODECYL SULFATE[J].Acta Veterinaria et Zootechnica Sinica,2001,32(3):249-252. |
| |
| Authors: | Yu Shengqing Liu Xiaowen XU Bu Ding Chan |
| |
| Abstract: | Through the sodium dodecyl sulfate—polyacrylamide gel electrophoresis we analysed and compared the structural proteins of 9 Mycoplasma gallisepticum strains，including type strain S6，F，and 6 field isolates from China．The result showed that the protein profiles of all these strains were different，the p64 was expressed in higher amounts in vitro in MG strains considered more virulent (S6 and R) than in MG strains of reduced virulence (F，et al)．Protein profiles showed similarities of S6 and R．F，D9601，D9603 and D9605 all had a spe cial 75 kDa band，but all virulent strains had not．The result suggests that the virulent field isolates in China maybe diversified． |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
