| 高活性苎麻脱胶菌的选育 |
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| 引用本文： | 王清,曾莹,阳飞.高活性苎麻脱胶菌的选育[J].农产品加工．学刊,2010(1):49-52. |
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| 作者姓名： | 王清 曾莹 阳飞 |
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| 作者单位： | 湖北工业大学生物工程学院,湖北,武汉,430068 |
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| 摘 要： | 苎麻生物法脱胶是一种绿色环保的脱胶方法,具有脱胶质量稳定和不损伤纤维的特点。为了选育稳定、高活性苎麻脱胶菌,以透明圈法和摇瓶发酵法从土壤中分离初筛得到的微生物脱胶菌株(15号)作为出发菌株,采用紫外和亚硝酸复合诱变处理,以青霉素抗性为标记,对大量初筛得到的突变株进行筛选,最终选育得到4株高活性苎麻脱胶菌株。与出发菌株相比,其中U-4使木聚糖酶活提高了15.81%,果胶酶活提高了6.49%,纤维素酶活降低了37.45%,残胶率降低了16.89%。
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| 关 键 词： | 苎麻 青霉素抗性 复合诱变 残胶率 |
Screening Strain of Ramie with Highly Activity Degumming |
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| Wang Qing,Zeng Ying,Yang Fei.Screening Strain of Ramie with Highly Activity Degumming[J].Nongchanpin Jlagong.Xuekan,2010(1):49-52. |
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| Authors: | Wang Qing Zeng Ying Yang Fei |
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| Institution: | Bioengineering College/a>;Hubei University of Technology/a>;Wuhan/a>;Hubei 430068/a>;China |
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| Abstract: | The biological degumming of ramie bast fibers is a green and envionment-friendly kind of method,which has the advantages of stable quality and no fiber damage. To screening stable and high activity strains,in this experiment we take a method of transparent circle and then bottle fermentation. The bacterial degumming strain named 15 which was screened and separated from soil was used for the original strain,By using compound mutation treatment of Ultraviolet and HNO2,and marked with penicillinr,we finally sc... |
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| Keywords: | ramie penicillin compound mutation remnant pectin rate |
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