| 低温胁迫对3个小白菜OguCMS与保持系生化指标的影响 |
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| 引用本文： | 安林海,陈龙正,胡俏强,徐海,宋波,苏小俊,袁希汉.低温胁迫对3个小白菜OguCMS与保持系生化指标的影响[J].中国蔬菜,2010,1(4):19-23. |
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| 作者姓名： | 安林海 陈龙正 胡俏强 徐海 宋波 苏小俊 袁希汉 |
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| 作者单位： | 1.南京农业大学，江苏南京 210095；;2.江苏省农业科学院蔬菜研究所，江苏南京 210014 |
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| 基金项目： | 国家科技支撑计划(2006BAD13B06,2006BAD01A7); 国家高技术研究发展计划(2008AA10Z150); 公益性行业(农业)科研专项经费项目(nyhyzx07-007) |
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| 摘 要： | 以3个小白菜OguCMS与保持系为试材,苗期进行低温处理,对其叶绿素、可溶性糖、可溶性蛋白质和脯氨酸含量及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化进行研究。结果表明:不育系的叶绿素、可溶性糖、可溶性蛋白质和脯氨酸含量在处理的各阶段均低于其保持系,SOD、POD和CAT活性则是不育系高于其保持系。随着低温处理时间的延长,不育系和保持系叶绿素和可溶性蛋白质含量一直下降,可溶性糖和脯氨酸含量呈现先上升后下降的趋势;SOD、POD和CAT活性呈现先上升后下降的趋势。不育系和保持系在处理的各个阶段变化的一致性和处理后各生化指标数值差异缩小,表明小白菜OguCMS在苗期遇低温的抗逆能力与保持系很接近。
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| 关 键 词： | 小白菜 OguCMS 低温胁迫 生化指标 |
| 收稿时间： | 2009-08-28; |
Effects of Cold Stress to Three Non-heading Chinese Cabbage OguCMS and Biochemical Indexes of Its Maintaining Line |
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| AN Lin-hai,CHEN Long-zheng,HU Qiao-qiang,XU Hai,SONG Bo,SU Xiao-jun,YUAN Xi-han.Effects of Cold Stress to Three Non-heading Chinese Cabbage OguCMS and Biochemical Indexes of Its Maintaining Line[J].China Vegetables,2010,1(4):19-23. |
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| Authors: | AN Lin-hai CHEN Long-zheng HU Qiao-qiang XU Hai SONG Bo SU Xiao-jun YUAN Xi-han |
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| Institution: | 1.Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;2.Vegetable Research Institute,Jiangsu Academy of Agricultural Sciences,Nanjing,210014,Jiangsu,China |
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| Abstract: | Three cytoplasmic male sterile lines(CMS)and their maintainer lines of the non heading Chinese cabbage〔Brassica campestris L.ssp.chinensis（L.）Makino var.communis Tsen et Lee〕.were taken as experimental materials,and the contents of chlorophyll,soluble sugar,soluble protein,proline and changes of the activities of SOD,POD,CAT in leaf were tested during seedling.The results showed that the contents of chlorophyll,soluble sugar,soluble protein,proline in CMS were significant lower than their maintainer,while the activities of three protective enzymes(SOD,POD and CAT)were higher in CMS with the treatment of low temperature stress.The contents of chlorophyll and soluble protein decreased continuously in both CMS and their maintainer;while the contents of soluble sugar,praline and the activities of three protective enzymes(SOD,POD and CAT)at first increased,then decreased under cold-stress.There was consistency in all stages of change between CMS and its maintainer line.And after processing,the differences in physiological and biochemical characteristics reduced.All these facts suggested the resistance of cold stress of non-heading Chinese cabbage OguCMS was greatly improved. |
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| Keywords: | Non-heading Chinese cabbage OguCMS Cold stress Biochemical index |
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