| 传染性造血组织坏死症病毒囊膜蛋白在原核细胞中的表达 |
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| 引用本文： | 郭露玲,刘海滨,石剑,李谷,肖庚富.传染性造血组织坏死症病毒囊膜蛋白在原核细胞中的表达[J].华中农业大学学报,2009,28(2). |
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| 作者姓名： | 郭露玲 刘海滨 石剑 李谷 肖庚富 |
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| 作者单位： | 1. 华中农业大学水产学院,武汉,430070
2. 武汉大学生命科学学院/病毒学国家重点实验室,武汉,430072 |
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| 基金项目： | 国家重大科学研究项目 |
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| 摘 要： | 通过RT-PCR获得了传染性造血组织坏死症病毒(infectious hematopoietic necrosis virus,IHNV)囊膜糖蛋白(G蛋白)基因,并将其克隆入新型MBP(麦芽糖结合蛋白)融合表达质粒pMAL-c2X,构建重组质粒pMAL-c2X/IHNVG并转入大肠杆菌Rossetta-gami2(DE3)splyS.经IPTG诱导后.表达出包含全长G蛋白在内、预期分子质量为99 ku的融合蛋白.基于anti-MBP抗体的Western blot鉴定证实表达蛋白存在.
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| 关 键 词： | 传染性造血组织坏死症病毒 囊膜蛋白 基因克隆 原核表达 |
Expression of Full Length Glycoprotein Gene of Infectious Hematopoietic Necrosis Virus (IHNV) in Escherichia coli |
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| GUO Lu-ling,LIU Hai-bin,SHI Jian,LI Gu,XIAO Geng-fu.Expression of Full Length Glycoprotein Gene of Infectious Hematopoietic Necrosis Virus (IHNV) in Escherichia coli[J].Journal of Huazhong Agricultural University,2009,28(2). |
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| Authors: | GUO Lu-ling LIU Hai-bin SHI Jian LI Gu XIAO Geng-fu |
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| Abstract: | The full length sequence(about 1.5 kb) of IHNV-G gene was obtained by RT-PCR amplification.The amplified fragment was cloned into prokaryotic expression system pMAL-c2X vector and then was transformed into Rossetta-gami2(DE3)splysS.Recombinants were examined with restriction enzyme digestion and further the interest insert confirmed by sequencing pMAL-c2X-G plasmid.SDS-PAGE revealed that the expressed fusion protein is produced when induced with IPTG,and its molecular weight is around 99 ku,which is the exp... |
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