| 三七总皂甙的液相色谱分析及其稳定性研究 |
| |
| 引用本文： | 闫海全,李崇瑛.三七总皂甙的液相色谱分析及其稳定性研究[J].安徽农业科学,2009,37(13):5973-5974. |
| |
| 作者姓名： | 闫海全 李崇瑛 |
| |
| 作者单位： | 成都理工大学材料与化学化工学院,四川成都,610059;成都理工大学材料与化学化工学院,四川成都,610059 |
| |
| 摘 要： | 目的]建立三七总皂甙的液相色谱定量分析方法，研究三七总皂甙的稳定性。方法]以三七总皂甙为考察指标，采用高效液相色谱法，探讨不同pH值、温度对三七总皂甙稳定性的影响。色谱条件如下：色谱柱为KromasilC18（4．6mm×250mm，5μm）；流速为1．00ml／min；检测波长为197nm；柱温为室温，采用二元梯度洗脱，流动相为乙腈-水。结果]三七总皂甙液相色谱定量方法具有较强的针对性和准确性。三七总皂甙在pH值〉4，低温条件下，比较稳定，而在强酸高温下受影响较大，在pH值为3时，总皂甙含量降低约32．5％，在100℃下，总皂甙含量降低约12．5％。结论]三七总皂甙在弱酸弱碱，低温条件下，比较稳定，而在强酸高温下受影响大。
|
| 关 键 词： | 三七 总皂甙 高效液相色谱 稳定性 |
HPLC Analysis of Total Saponins of Panax notoginseng and Study on Its Stability |
| |
| YAN Hai-quan et al.HPLC Analysis of Total Saponins of Panax notoginseng and Study on Its Stability[J].Journal of Anhui Agricultural Sciences,2009,37(13):5973-5974. |
| |
| Authors: | YAN Hai-quan |
| |
| Institution: | YAN Hai-quan et al (College of Materials , Chemical Engineering,Chengdu University of Technology,Chengdu,Sichuan 610059) |
| |
| Abstract: | Objective] The study aimed to establish the quantitative analysis method with HPLC on total saponin of Panax notoginseng and research the stability of the total saponin.Method] With the total saponin of P.notoginseng as the investigated index,the HPLC method was used to discuss the effect of different pH value and different temperature on the stability of the total saponins of P.notoginseng.The chromatographic conditions were as follows: chromatographic column of Kromasil C18(4.6 mm×250 mm,5 μm),flow rate at 1.00 ml/min,detection wavelength at 197 nm and column temperature of room temperature,by using the mobile phase of acetonitrile-water with duality gradient elution.Result] The analytical method with HPLC quantitative measurement on total saponin of P.notoginseng was precise and accurate.The content of total saponin of P.notoginseng was stabile at pH>4 and low temperature,but that was greatly effected under the strong acid and high temperature.The content of P.notoginseng was decreased by nearly 32.5% at pH of 3 and by 12.5% at 100 ℃.Conclusion] The total saponin of P.notoginseng was stable under the weak acid and weak base and low temperature condition,but that was greatly affected under the strong acid and high temperature. |
| |
| Keywords: | Panax notoginseng Total saponin HPLC Stability |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
