| 分根装置中接种AM真菌对玉米秸秆降解及土壤微生物量碳、氮和酶活性的影响 |
| |
| 引用本文： | 罗 珍,朱 敏,王晓锋,刘先良,郭 涛.分根装置中接种AM真菌对玉米秸秆降解及土壤微生物量碳、氮和酶活性的影响[J].中国生态农业学报,2013,21(2):149-156. |
| |
| 作者姓名： | 罗 珍 朱 敏 王晓锋 刘先良 郭 涛 |
| |
| 作者单位： | 西南大学资源环境学院 重庆 400716;西南大学资源环境学院 重庆 400716;西南大学资源环境学院 重庆 400716;西南大学资源环境学院 重庆 400716;西南大学资源环境学院 重庆 400716 |
| |
| 基金项目： | 中央高校基本科研业务费专项(XDJK2010B012)和农业部公益性行业专项(201103003)资助 |
| |
| 摘 要： | 本试验通过两室分根装置种植玉米,利用网袋法研究接种Glomus mosseae和Glomus etunicatum两种AM真菌对玉米秸秆降解的影响。试验分别在玉米移栽后第20 d、30 d、40 d、50 d和60 d时取样,通过测定接种AM真菌后玉米秸秆中碳、氮释放,土壤中3种常见酶活性、微生物量碳、微生物量氮及土壤呼吸的动态变化,探讨AM真菌降解玉米秸秆可能的作用机制。研究结果表明:经60 d的培养后,与未接种根室相比,接种G.mosseae和G.etunicatum真菌的菌根室玉米秸秆降解量提高了20.75%和20.97%;另外,接种G.mosseae和G.etunicatum加快了玉米秸秆碳素释放,降低了氮素释放,致使碳氮比降低25.45%和26.17%,有利于秸秆进一步降解。在本试验条件下,接种AF真菌的菌根室中土壤酸性磷素酶、蛋白酶和过氧化氢酶活性均有显著提高,并增加了微生物量碳、氮和土壤呼吸作用,形成了明显有别于根际的微生物区系。这一系列影响都反映出AM真菌能够直接或间接作用于玉米秸秆的降解过程,是导致玉米秸秆降解加快的重要原因。
|
| 关 键 词： | 分根装置 玉米秸秆 秸秆降解 土壤酶活性 微生物量碳 微生物量氮 土壤呼吸 |
| 收稿时间： | 2012/7/30 0:00:00 |
| 修稿时间： | 2012/9/29 0:00:00 |
Influence of arbuscular mycorrhizal inoculation on maize straw degradation and soil microbial biomass carbon and nitrogen, and enzyme activity in di-chamber split-root device |
| |
| LUO Zhen,ZHU Min,WANG Xiao-Feng,LIU Xian-Liang and GUO Tao.Influence of arbuscular mycorrhizal inoculation on maize straw degradation and soil microbial biomass carbon and nitrogen, and enzyme activity in di-chamber split-root device[J].Chinese Journal of Eco-Agriculture,2013,21(2):149-156. |
| |
| Authors: | LUO Zhen ZHU Min WANG Xiao-Feng LIU Xian-Liang and GUO Tao |
| |
| Institution: | College of Resources and Environment, Southwest University, Chongqing 400716, China;College of Resources and Environment, Southwest University, Chongqing 400716, China;College of Resources and Environment, Southwest University, Chongqing 400716, China;College of Resources and Environment, Southwest University, Chongqing 400716, China;College of Resources and Environment, Southwest University, Chongqing 400716, China |
| |
| Abstract: | A large number of plant residues enters into soil on a daily basis in nature. The decomposition of these residues forms the foundation of nutrient cycles, especially carbon/nitrogen cycle. Several physical, chemical and biological factors (and interactions) contribute to the degradation processes. Among biological factors, micro-organisms which act as consumers and decomposers directly accelerate degradation processes or indirectly use acids and enzymes to do so. As micro-organisms, mycorrhizas have been recognized with special importance due to their especial microhabitat requirements. Arbuscular mycorrhizal (AM) fungi can form mutualistic symbiosis with more than 80% of higher plant species. The contribution of AM to plant residue degradation processes have varied at different hierarchical levels (plant root, mycorrhizas and soil mycelium), of course with mentioning accompanying bacteria. Most experiments have been carried out in pot or single compartment device conditions, which have made it difficult to clarify different effects of mycorhiza symbiosis on plant residue degradation. In this study, a di-compartment split-root device was used to quantitatively compare the changes in degradation processes in mycorrhizosphere and rhizosphere conditions. In the experiment, maize straw was used as representative plant residue and two different AM fungi (Glomus mosseae and Glomus etunicatum) inoculated. Samples were respectively harvested in 20 d, 30 d, 40 d, 50 d and 60 d after inoculation, analyzed for soil enzymatic activity, soil microbial biomass carbon and nitrogen, soil respiration and qCO2 and the mechanism of how mycorrhizal inoculation accelerated maize straw degradation discussed. The results showed that by inoculation with two different AM fungi, maize straw degradation mass and coefficient in mycorrhizosphere compartment were higher than in root compartment. Mycorrhizal inoculation enhanced carbon degradation but limited nitrogen degradation. It also decreased carbon/nitrogen ratio which facilitated further degradation. For soil biological performance, it was noted that catalase, protease, acid phosphatase, microbial biomass carbon, nitrogen and soil respiration were more enhanced in mycorrhizosphere compartment than in root compartment. This initiated the next step of forming more active microbial community. The increased indices involved in the degradation process were the main reasons behind mycorrhizal acceleration of degradation. Different ability to accelerate maize straw degradation lied in function diversity of AM fungi. More AM fungal species and soil types were suggested for consideration in future studies. |
| |
| Keywords: | Split-root device Maize straw Straw decomposion Soil enzyme activity Microbial biomass carbon Microbial biomass nitrogen Soil respiration |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国生态农业学报》浏览原始摘要信息 |
| 点击此处可从《中国生态农业学报》下载免费的PDF全文 |
|
