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湖南省葡萄扇叶病调查及检测研究
引用本文:周敏,;聂松青,;罗奕凡,;刘昆玉,;舒广平,;邓子牛,;李大志.湖南省葡萄扇叶病调查及检测研究[J].湖南农业科学,2014(4):50-52.
作者姓名:周敏  ;聂松青  ;罗奕凡  ;刘昆玉  ;舒广平  ;邓子牛  ;李大志
作者单位:[1]国家柑橘改良中心长沙分中心,湖南长沙410128; [2]湖南农业大学园艺园林学院,湖南长沙410128; [3]湖南省葡萄工程技术研究中心,湖南长沙410128; [4]湖南省园艺研究所,湖南长沙410125
基金项目:农业部公益性行业(农业)科研专项(201203076-06);湖南省研究生科技创新基金(54040112029-CX20128301)
摘    要:对湘西北、湘南、湘中和湘西等葡萄主产区的扇叶病发病及危害情况进行了调查,并通过生物学和分子生物学的方法对各地区的疑似病株进行了检测。结果表明:在湖南的葡萄主产区,葡萄扇叶病的感病品种较多,在夏黑无核、红地球、金星无核和金手指等品种上均有发生,但湘西的刺葡萄表现出对葡萄扇叶病有一定的抗性;感病症状总体上表现为叶片褪绿、黄化,叶缘锯齿变尖锐或形状不规则,并严重影响葡萄植株的生长,导致树势减弱、产量下降、果实品质变差;生物学检测结果显示,采集的54个样品,其中有52%的样品在千日红和本氏烟草上表现出系统性斑驳和变形;而分子生物学检测结果显示,54个样品中有29个样品检测到葡萄扇叶病病毒,以金星无核和红地球两个品种的检出率较高。

关 键 词:葡萄扇叶病  调查  检测  湖南省

Survey and Detection of the Grape Fanleaf Disease in Hunan Province
Institution:ZHOU Min, NIE Song-qing, LUO Yi-fan, LIU Kun-yu, SHU Guang-ping, DENG Zi-niu, LI Da-zhi (1. Changsha Branch of National Center for Cimzs Improvement, Changsha 410128, PRC; 2. College oHorticulmre and Landscape, HNA U, Changsha 410128, PRC; 3. Hunan Provincial Engineering and Technology Research Center for Grapes, Changsha 410128, PR C; 4. ttunan Horticultural Research Institute, Ch an gsha 410125, PR C)
Abstract:The occurrence situation and damages of grape fanleaf disease in main grape production areas in Hunan province, such as northwest Hunan, southern Hunan, middle Hunan and western Hunan, were investigated, and the suspected disease plants from each area were detected by biological method and molecular biological method. The results showed that in main grape production areas of Hunan province, many grape varieties occurred grape fanleaf disease, such as Xiahei-seedless, Red-earth, Jinxing-seedless, Golden-finger, etc., but the Vitis davidii var. davidii from western Hunan showed a certain resistance to the grape fanleaf disease. The symptoms of the grape fanleaf disease generally showed as follows: the leaf was degreening and yellowing; the serrate leaf margin turned to sharpening or out-of-shape; the growth of grape plants was influenced seriously, thus weakening tree vigour, reducing yield and decreasing fruit quality. The biological detection results indicated that among 54 collected samples, 52% samples presented systemic mottle and distortion in Qianrihong and Benshi tobacco varieties. Moreover, the molecular biological detection results revealed that 29 out of 54 samples detected grape fanleaf disease virus, and the detection rate of the Jinxing-seedless and the Red-earth was relatively high.
Keywords:grape fanleaf disease  survey  detection  Hunan province
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