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| 转Bt基因杨树对美国白蛾幼虫中肠解毒酶及乙酰胆碱酯酶的影响 | |
| 引用本文： | 丁双阳,李怀业,李学锋,张志毅.转Bt基因杨树对美国白蛾幼虫中肠解毒酶及乙酰胆碱酯酶的影响[J].东北林业大学学报,2001,29(3):28-30. |
| 作者姓名： | 丁双阳 李怀业 李学锋 张志毅 |
| 作者单位： | 1. 北京林业大学, 2. 辽宁省清原县林业局森保站 3. 中国农业大学应用化学学院 4. 北京林业大学 |
| 基金项目： | 国家博士后基金资助项目 |
| 摘 要： | 以转Bt基因欧洲黑杨叶片饲喂4-5龄美国白蛾幼虫，对其中肠几种重要解毒酶及乙酰胆碱酯酶的活性进行了测定。结果表明，中肠多功能氧化酶受到强烈抑制，饲喂4h后，多功能氧化酶的活性比对照降低50.89%，48h后，该酶活性仅为对照的1/6，酯酶及羟酸酯酶的活性也受到明显抑制，饲喂48h后，上述两种酶的活性分别比对照降低73.58%和56.55%，谷胱甘肽S-转移酶变化比较复杂，饲喂24h后其活性出现峰值；48h后与对照持平，中肠乙酰胆碱酯酶活性无明显变化，转基因叶片对幼虫中肠解毒酶活性的抑制可能是其毒杀害虫的作用机制之一。 |
| 关 键 词： | 转Bt基因杨树 美国白蛾 解毒酶 乙酰胆碱酯酶 |
Effects of Bt Transgenic Poplar on Detoxification Enzyme and AChE in American White Moth Larvae |
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| Ding Shuangyang.Effects of Bt Transgenic Poplar on Detoxification Enzyme and AChE in American White Moth Larvae[J].Journal of Northeast Forestry University,2001,29(3):28-30. | |
| Authors: | Ding Shuangyang |
| Institution: | Ding Shuangyang(Bejing Forestry University. Beijing 100083. P. R. China); Li Huaiye(Qingyuan Forestry Bureau. Liaoning Province): Li Xuefeng(China Agricultural University);Zhang Zhiyi(Beijing Forestry University) |
| Abstract: | The activities of several detoxification enzymes and acetylcholinesterase (AChE) of American white moth fourth-fifth instar larvae feeding on leaves of Bt trangenic poplar (Populus nigra L. ) were investigated. The results showed that the activity of mix-function oxidase in the midgut of tile larvae was decreased by 50. 89. % after 4 hours and 48 hours later it was only l/6 of control. The activities of esterase and carboxylesterase (CarE) tvers also decreased by 73. 58% and 56. 55%. The activity of AChE did not change obviously. The activity of glutathione s-transferase (GST) increasd remarkably after 24 hours feeded with trangenic poplar. Thus the detoxicification enzyme System in the midgut was inhibited and further toxic reaction of Bt protein could take place. This may be one of tile important mechanisms of Bt trangenic poplar. |
| Keywords: | Transgenic poplar American white moth Detoxification enzymes |
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