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| 半海水筛选耐盐紫花苜蓿细胞系的生理特性分析 | |
| 引用本文： | 姜健,杨宝灵,封德全,邹福海,李春斌,李海山.半海水筛选耐盐紫花苜蓿细胞系的生理特性分析[J].西北农业学报,2007,16(5):49-54. |
| 作者姓名： | 姜健 杨宝灵 封德全 邹福海 李春斌 李海山 |
| 作者单位： | 1. 大连民族学院生命科学学院,辽宁大连,116600 2. 大连市林业局,辽宁大连,116600 3. 本溪县种子管理站,辽宁本溪,117100 |
| 基金项目： | 辽宁省大连市科技局资助项目;大连民族学院校科研和教改项目 |
| 摘 要： | 以紫花苜蓿无菌苗下胚轴为外植体,诱导获得愈伤组织(M0)。经甲基磺酸乙酯(EMS)处理,通过一步筛选获得了耐半海水w(NaCl≈1.5%,1/2海水)的耐盐细胞系(Ms1),一部分耐盐细胞系转移到无盐培养基上,继代培养10代后得到新细胞系(Ms2)。无论是在无盐还是不同程度盐胁迫条件下,Ms1的成活率、生长率、脯氨酸含量、Cl-、K 、K /Na 、可溶性蛋白质含量都高于M0,差异显著。Ms2介于两者之间,但是与Ms1基本接近,差异不显著。在w(NaCl≈0.6%,1/5海水)条件,M0细胞系的SOD活性高于Ms1和Ms2;在w(NaCl≈1%,1/3海水)条件下,Ms1、Ms2细胞系的SOD活性达到最高,与M0可溶性蛋白质SDS-PAGE图谱相比较,Ms1、Ms2中出现两条新多肽带(P1=58kD,P2=26kD),这两条多肽带可能与耐盐性有关。 |
| 关 键 词： | 紫花苜蓿 耐盐细胞系 半海水培养基 愈伤组织 生理生化特性 |
| 文章编号： | 1004-1389（2007）05-0049-06 |
| 收稿时间： | 2006-09-21 |
| 修稿时间： | 2007-05-31 |
Physiological Characteristics Analysis of Salt-tolerant Cell Line from Alfalfa Callus Cultures Selected on Half Natural Seawater Medium |
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| JIANG Jian,YANG Bao-ling,FENG De-quan,ZOU Fu-hai,LI Chunbin and LI Hai-shan.Physiological Characteristics Analysis of Salt-tolerant Cell Line from Alfalfa Callus Cultures Selected on Half Natural Seawater Medium[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(5):49-54. | |
| Authors: | JIANG Jian YANG Bao-ling FENG De-quan ZOU Fu-hai LI Chunbin and LI Hai-shan |
| Institution: | 1. College of Life Science,Dalian Nationalities University,Dalian Liaoning 116600,China; 2. Dalian Forestry Bureau, Dalian Liaoning 116600,China; 3. Seed Office, Agricultural Bureau of Benxi County, Benxi Liaoning 117100,China |
| Abstract: | M0 cell line of alfalfa(Medicago sativa L.)was induced from alfalfa aseptic hyopocotyl segments.Salt-tolerant cell line(Ms1)of alfalf a tow(NaCl 1.5%,half seawater)was obtained directly after dealing with EMS.After some Ms1 was subcultured through 25 passages in a medium containing half natural seawater,then transfer red to NaCl free medium for 10 passages and the Ms2 cell line was gained.The results of physiological character istics analysis showed,whether normal condition or salt stress,the survival rate of calli(SRC),growth rate of calli(GRC),the contents of proline,K+,and Cl-,the rate of Ms1 and Ms2 were significantly higher than those of M0.In despite of above indexes of Ms2 were between M0 and Ms1,but all were near to Ms1,and there was no remarkable difference with Ms1.In normal condition,the SOD activity and Na+ of M0 were lower than Ms1 and Ms2.In condition of lower seaw ater concentration,the SOD activity and Na+ of M0 were higher than Ms1 and Ms2.SDS-PAGE electrophoresis of soluble proteins indicated that there were two new protein bands in Ms1 and Ms2 compared to the control.These proteins were 58kD and 26kD and they might be related to salt tolerant. |
| Keywords: | Medicago sativa Salt-tolerant cell line Half seawater medium Callus Physiological and biochemical characteristics |
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