| 大孔吸附树脂对杜仲叶中绿原酸、总黄酮的分离研究 |
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| 引用本文： | 马希汉,王冬梅,苏印泉.大孔吸附树脂对杜仲叶中绿原酸、总黄酮的分离研究[J].林产化学与工业,2004,24(3):47-51. |
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| 作者姓名： | 马希汉 王冬梅 苏印泉 |
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| 作者单位： | 1. 西北农林科技大学,生命科学学院,陕西,杨陵,712100
2. 西北农林科技大学,陕西省经济植物资源开发利用重点实验室,陕西,杨陵,712100 |
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| 基金项目： | 国家“十五”科技攻关项目 (2 0 0 1BA5 0 2B0 4 0 3) |
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| 摘 要： | 通过对静态吸附容量和洗脱效果的选择，从10种大孔吸附树脂中确定出最适于杜仲叶中绿原酸、总黄酮分离的XDA-5树脂。研究了用该树脂吸附分离杜仲叶中绿原酸和总黄酮的方法，得出以下结论：1)杜仲叶绿原酸和总黄酮的最佳分离工艺为：上柱液pH值为2-3，静态吸附时间8h，绿原酸最佳洗脱剂为10％～15％乙醇溶液，黄酮洗脱剂为50％-70％乙醇溶液，流速为每分钟流出液体积相当于吸附剂体积的8％；2)10％～15％乙醇洗脱液经真空浓缩后，其固形物得率为4．85％，绿原酸含量为36．65％；3)50％～70％乙醇洗脱液经真空浓缩后，其固形物得率为4．98％，总黄酮含量为28．34％。
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| 关 键 词： | 杜仲叶 绿原酸 总黄酮 树脂 |
| 文章编号： | 0253-2417(2004)03-0047-05 |
STUDY ON ISOLATION OF CHLOROGENIC ACID AND FLAVONOIDS IN EUCOMMIA ULMOIDES LEAF BY MACROPOROUS RESIN |
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| MA Xi-han,WANG Dong-mei,SU Yin-quan.STUDY ON ISOLATION OF CHLOROGENIC ACID AND FLAVONOIDS IN EUCOMMIA ULMOIDES LEAF BY MACROPOROUS RESIN[J].Chemistry & Industry of Forest Products,2004,24(3):47-51. |
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| Authors: | MA Xi-han WANG Dong-mei SU Yin-quan |
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| Institution: | MA Xi-han~1,WANG Dong-mei~2,SU Yin-quan~2 |
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| Abstract: | A specific macroporous resin,XDA-5 was selected from 10 different resins to adsorb and isolate chlorogenic acid and flavonoids from the leaves of Eucommia ulmoides through investigating the factors such as the capability of static adsorption and desorption.Thereafter,a methodological study was carried out on this resin.The following results were obtained.1)The favorable technological parameters for the isolation are:pH value 2-3 of the liquid extract to be isolated,static adsorption time 8 h,eluant for chlorogenic acid 10%-15% ethanol,for flavonoids 50%-70% (ethanol),elution rate 8% of the volume of the adsorbent per minute;2)The eluent rich in chlorogenic acid is concentrated by vacuum distillation at a yield of solid extracts 4.85%,content of chlorogenic acid in solid extract 36.65%,yield of chlorogenic acid 1.78%;3)The eluent rich in flavonoids is concentrated at a yield of solid extracts 4.98%,content of flavonoids 28.34%. |
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| Keywords: | Eucommia ulmoides leaf chlorogenic acid flavonoids resin |
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