| 甘蓝型油菜遗传图谱的构建及开花期的QTL分析 |
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| 引用本文： | 蔡长春,傅廷栋,陈宝元,涂金星.甘蓝型油菜遗传图谱的构建及开花期的QTL分析[J].中国油料作物学报,2007,29(1):1-8. |
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| 作者姓名： | 蔡长春 傅廷栋 陈宝元 涂金星 |
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| 作者单位： | 华中农业大学作物遗传改良国家重点实验室，武汉，430070
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| 基金项目： | 国家自然科学基金;教育部长江学者和创新团队发展计划 |
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| 摘 要： | 在由两个春性甘蓝型油菜双低品种DH401（早花）和Q2（迟花）的F1代植株通过小孢子培养所获得的DH（doubled haploid）群体中，应用SSR、SRAP及AFLP标记构建了一张遗传连锁图谱，并对开花期性状进行了数量性状座位（QTL）分析。在亲本间共检测到263个有多态性的遗传标记，其中SSR标记有88个、SRAP标记101个及AFLP标记74个。其中248个标记分布于19个连锁群，总遗传距离为1634.7 cM，标记间平均遗传距离为6.6 cM，标记偏分离比例达到27.4% (p<0.01)且主要集中在第4、5连锁群。应用QTLMAPPER 1.6在武汉、和政分别检测到2个和4个控制开花期的主效QTL位点，分别解释了68.63%和75.83的开花期表型变异，其中有2个主效QTL位点在这两地同时被检测到。另外也分析了影响开花期的上位效应并探讨了本研究结果在实际育种中的意义。
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| 关 键 词： | 甘蓝型油菜 开花期 连锁图谱 数量性状座位（QTL） |
| 文章编号： | 1007-9084（2007）01-0001-08 |
| 修稿时间： | 2006-05-18 |
Construction of a genetic linkage map and its use for QTL analysis of flowering time in Brassica napus L. |
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| CAI Chang-chun,FU Ting-dong,CHEN Bao-yuan,TU Jin-xing.Construction of a genetic linkage map and its use for QTL analysis of flowering time in Brassica napus L.[J].Chinese Journal of Oil Crop Sciences,2007,29(1):1-8. |
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| Authors: | CAI Chang-chun FU Ting-dong CHEN Bao-yuan TU Jin-xing |
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| Institution: | National Key Laboratory of Crop Genetics Improvement ,Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | A genetic linkage map composed of simple sequence repeat(SSR) marker,sequence-related amplified polymorphism(SRAP) marker and amplified fragment length polymorphism(AFLP) marker was built using one DH(doubled haploid) population derived from an F1 between two spring Brassica napus cultivars,early-flowering DH401 and late-flowering Q2,by microspore culture.QTL analysis of flowering time was performed in this DH population.A total of 263 markers including 88 SSR marker loci,101 SRAP marker loci and 74 AFLP marker loci were detected.Out of them,248 markers were assembled into 19 linkage groups (N1~N19).The remaining 15 markers,could not be assembled to any linkage groups.The total map length was 1 634.7cM with an average distance of 6.6cM and a 27.4% of distortion segregation(p<0.01) of markers mainly clustering in N4 and N5.Two and four major-effect QTLs controlling flowering time were identified,which explained 68.63% and 75.83% of phenotype variation in Wuhan and Hezheng,respectively.Moreover,two major-effect QTLs were simultaneously detected in the two locations.In addition epistatic interactions conditioning flowering time were also analyzed.The implications of the results in this study were discussed. |
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| Keywords: | Brassica napus flowering time linkage map QTL analysis |
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