In vitro morphogenesis of juvenile Annona cherimola Mill,bud explants |
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| Authors: | C L Encina A Barceló-Muñoz A Herrero-Castaño F Pliego-Alfaro |
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| Institution: | 1. Estación Experimental La Mayora, C.S.I.C., 29750 Algarrobo-Costa, Málaga, Spain;2. Centro de Investigación y Desarrollo Agrario, 29140 Churriana, Málaga, Spain;3. Departamento de Biología Vegetal, Universidad de Málaga, Campus de Teatinos s/n, 29071 Málaga, Spain (Corresponding author) |
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| Abstract: | A micropropagation procedure for juvenile cherimoya (Annona clierimola Mill.) is described. Axillary shoot proliferation was obtained after culturing nodal sections with lateral buds in basal medium supplemented with either 0.66 μM BA or 1,36μM zeatin. For root induction shoots were pre-incubated for 7 d in basal medium supplemented with 1 gl?1 activated charcoal, then cultured for 10 d (7 dark/3 light) on medium with 500 μM IBA, 58.4 mM sucrose, and 200 mg I“1 citric acid. Afterwards, shoots were transferred to the same medium without auxin and with the macroelements at half strength. Using this procedure, 95% of shoots rooted. The survival rate at the end of the acclimatization period was 70%. |
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