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| 一种高质量葡萄基因组DNA提取方法 | |
| 引用本文： | 徐美隆,章雨,倪细炉.一种高质量葡萄基因组DNA提取方法[J].北方园艺,2011(15):172-174. |
| 作者姓名： | 徐美隆 章雨 倪细炉 |
| 作者单位： | 1. 国家经济林木种苗快繁工程技术研究中心,宁夏银川,750004 2. Center for Grapevine Biotechnology,William H. Darr School of Agriculture,Missouri State University,Mountain Grove,MO,USA 65711 3. 种苗生物工程国家重点实验室,宁夏银川,750004 |
| 基金项目： | 宁夏回族自治区科技攻关项目国际科技合作专项资助项目; 宁夏自然科学基金资助项目(NZ09193) |
| 摘 要： | 针对葡萄组织中多酚、多糖类物质含量较高的特点,对比研究了利用植物总DNA提取试剂盒和改良CTAB法提取葡萄总DNA.结果表明:改良的CTAB法能够从不同葡萄品种和不同生长时期的叶片中获得高质量、完整性好的总DNA,其OD260/OD260介于1.7～1.9之间,无降解现象,RNA去除干净,完全适于进行PCR和酶切等研究. |
| 关 键 词： | 葡萄 DNA提取 PCR 酶切 |
A High-quality Extraction Method for Genomic DNA of Grapevine |
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| XU Mei-long , ZHANG Yu , NI Xi-lu.A High-quality Extraction Method for Genomic DNA of Grapevine[J].Northern Horticulture,2011(15):172-174. | |
| Authors: | XU Mei-long ZHANG Yu NI Xi-lu |
| Institution: | XU Mei-long1,ZHANG Yu2,NI Xi-lu3(1.The National Center of Research and Engineering Technology of Economic Forest Tree Speedy Propagation,Yinchuan,Ningxia 750004,2.Center for Grapevine Biotechnology,William H.Darr School of Agriculture,Missouri State University,Mountain Grove,MO 65711,3.The State Key Laboratory of the Seedling Bioengineering,Ningxia 750004) |
| Abstract: | Extracting high-quality DNA from grapevine tissues were particularly difficult due to high levels of polyphenolics and polysaccharides,commercial total DNA extraction kit and a improved CTAB method were applied to isolate DNA.The results showed that the improved CTAB method isolate high purity and integrity DNA from different varieties and different growing stages of the leaves.The ratio of OD260/OD280=1.7~1.9.Non-degradation phenomenon took place,and RNA removed clearly.The DNA was suitable for PCR and enz... |
| Keywords: | grapevine isolating DNA PCR enzyme digestion |
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