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萝卜雄性不育系花蕾发育过程中内源激素分析
引用本文:孙希禄,许小勇,张鲁刚.萝卜雄性不育系花蕾发育过程中内源激素分析[J].北方园艺,2011(19):11-15.
作者姓名:孙希禄  许小勇  张鲁刚
作者单位:西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源与遗传改良重点开放实验室,陕西杨凌712100
基金项目:国家自然科学基金资助项目(30871717); 国家科技支撑计划资助项目(2008BADB1B01)
摘    要:利用间接酶联免疫(ELISA)法测定了萝卜雄性不育株及可育株花蕾发育过程中内源激素含量的动态变化,以揭示萝卜雄性不育发生与其内源激素的关系.结果表明:在花蕾的发育过程中,不育株和可育株内源激素IAA、ABA、GA3、ZR以及JA含量变化均差异明显,在不育发生的初始阶段,不育株内源IAA含量上升32.09%,ABA含量下降75.45%,JA含量下降了11.20%,其余2种内源激素含量差异很小.IAA/ABA、IAA/ZR、IAA/GA3和GA3/ABA在二者间的变化趋势不一致,且比值大小差异很大,在不育发生的初始阶段,IAA/ABA、IAA/ZR和GA3/ABA平衡失调.表明内源激素可能与萝卜雄性不育的发生密切相关.

关 键 词:萝卜  雄性不育  花蕾  植物激素

Analysis on the Changes of Phytohormones During Flower Bud Development in Male-sterile Radish
SUN Xi-lu , XU Xiao-yong , ZHANG Lu-gang.Analysis on the Changes of Phytohormones During Flower Bud Development in Male-sterile Radish[J].Northern Horticulture,2011(19):11-15.
Authors:SUN Xi-lu  XU Xiao-yong  ZHANG Lu-gang
Institution:SUN Xi-lu,XU Xiao-yong,ZHANG Lu-gang(College of Horticulture,Northwest Agriculture and Forestry University,Key Laboratory of Horticulture Plant Germplasm Resource Utilization in Northwest China,Yangling,Shaanxi 712100)
Abstract:The changes of the contents of endogenous phytohormones were measured by ELISA(enzymelinked immunosorbent assay)to analyse the dynamic changes of endogenous phytohormones in different development stages of buds between male sterility and fertile radish.The results showed that the change of the phytohormones contents was significant during the whole bud development stages in male fertile and sterile plants at the development stage when the sterile phenomenon begin to appear,the content of IAA in male sterile...
Keywords:radish  male sterility  flower buds  phytohormone  
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