| 响应面优化虎杖固态发酵工艺条件的研究 |
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| 引用本文： | 王卫,黎继烈,曾柏全,姚跃飞.响应面优化虎杖固态发酵工艺条件的研究[J].北方园艺,2011(13):164-167. |
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| 作者姓名： | 王卫 黎继烈 曾柏全 姚跃飞 |
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| 作者单位： | 中南林业科技大学,生命科学学院,湖南,长沙,410004;中南林业科技大学,生命科学学院,湖南,长沙,410004;中南林业科技大学,生命科学学院,湖南,长沙,410004;中南林业科技大学,生命科学学院,湖南,长沙,410004 |
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| 基金项目： | 中南林业科技大学青年科学研究基金资助项目(07036B) |
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| 摘 要： | 应用产β-葡萄糖苷酶的黑曲霉固态发酵酶解虎杖中的虎杖苷,以提高虎杖中白藜芦醇的含量。采用均匀实验设计和响应面分析方法对影响虎杖中白藜芦醇含量的固态堆积发酵工艺条件(温度、湿度、菌龄和接种量)进行优化。结果表明:最佳的固态发酵工艺条件为:温度42℃,湿度1.85∶1、菌龄42h、接种量1.8%,在此条件下,发酵48h后,白藜芦醇含量提高为1.52%,是未进行固态发酵虎杖中白藜芦醇含量的4.47倍。优化固态发酵工艺条件能较大程度的提高从虎杖中获取白藜芦醇的得率。
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| 关 键 词： | 白藜芦醇 虎杖 虎杖苷 响应面 固态发酵 |
Study on the Optimization Technology of Polygonum cuspidatum in the Solid State Fermentation by Response Surface Methodology |
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| WANG Wei,LI Ji-lie,ZENG Bo-quan,YAN Yue-fei.Study on the Optimization Technology of Polygonum cuspidatum in the Solid State Fermentation by Response Surface Methodology[J].Northern Horticulture,2011(13):164-167. |
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| Authors: | WANG Wei LI Ji-lie ZENG Bo-quan YAN Yue-fei |
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| Institution: | WANG Wei,LI Ji-lie,ZENG Bo-quan,YAN Yue-fei(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha,Hunan 410004) |
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| Abstract: | With the polydatin hydrolysis by β-glucosidase produced by Aspergillus niger in the microbial solid state fermentation,the content of resveratrol in Polygonum cuspidatum can be increased.The influential factors,including temperature,humidity,strain age and inoculum,were investigated by uniform test design;the optimum technological conditions were obtained by regression and response surface analysis.The results showed that the best technological condition of solid state fermentation was as follows:temperature 42℃;humidity ratio 1.85∶1;strain age 42 h;inoculum 1.8%.Under such condition,after fermentation 48 h,resveratrol content in Polygonum cuspidatum raised to 1.52%,was nearly 4.47 times than that in Raw materials.With the optimization solid-state fermentation process,the resveratrol yield in the extraction of Polygonum cuspidatum would be greatly improved. |
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| Keywords: | resveratrol Polygonum cuspidatum polydatin response surface methodology solid state fermentation |
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