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Prepro-orexin基因多样性与猪产仔数的相关性
引用本文:管峰,罗媛媛,刘光富.Prepro-orexin基因多样性与猪产仔数的相关性[J].中国农学通报,2007,23(8):36-39.
作者姓名:管峰  罗媛媛  刘光富
作者单位:1. 中国计量学院生命科学学院,杭州,310018
2. 中国计量学院计量技术工程学院,杭州,310018
基金项目:浙江省自然科学基金(Y305107)和中国计量学院基金项目(XZ0538).
摘    要:为了研究Prepro-orexin基因多样性与繁殖性能的关系,确立其在分子标记育种中的应用价值。采用PCR-RFLP法分析猪Prepro-orexin基因第62位多态性位点在二花脸、香猪、淮猪和姜曲海猪4个品种中的多样性,并分析其不同基因型对二花脸猪产仔数的影响。结果表明,二花脸和香猪分别检出三种基因型,姜曲海猪有两种基因型,而淮猪只有一种基因型。各等位基因频率和基因型频率在不同品种间存在显著差异;二花脸猪不同基因型群体间产仔数差异不显著,说明该基因多样性对产仔数的影响不显著。

关 键 词:  Prepro-orexin基因  基因多态性  产仔数
修稿时间:2007-04-242007-06-08

Polymorphism of Pig Prepro-orexin Gene and Its Relationship on Litter Size
Guan Feng,Luo Yuanyuan,Liu GuangFu.Polymorphism of Pig Prepro-orexin Gene and Its Relationship on Litter Size[J].Chinese Agricultural Science Bulletin,2007,23(8):36-39.
Authors:Guan Feng  Luo Yuanyuan  Liu GuangFu
Institution:1College of life sciences, China Jiliang University, Hangzhou 310018;2College of Metrological Technology and Enginee ring; Jiliang University, Hangzhou, 310018
Abstract:In this experiment, polymorphism of 62nd SNP of Prepro-orexin gene in four porcine breeds (including Erhualian, xiang, huai and Jiangquhai pig) was anylised by PCR-RFLP method. The relationship between litter size and different genotype of Erhualian breed was analysized. The results showed that there are three genptypes in four porcine breeds, including AA, AB and BB. Frequencies of allele and genotype had a significant difference in differenf breeds. Erhualian and Xiang pigs had three genotypes, Jiangquhai pig had two genotypes, and Huai had only AA genotype. Litter size in populations with differenf genotypes had no significant difference within Erhualian pig, which showed that polymorphism of 62nd SNP of Prepro-orexin gene had no effect on litter size.
Keywords:Porcine  Prepro-orexin gene  Litter size
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