| 发掘大豆资源中灰斑病1号生理小种抗性优异等位变异 |
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| 引用本文： | 王财金,弟文静,马淑梅,王洋.发掘大豆资源中灰斑病1号生理小种抗性优异等位变异[J].作物杂志,2020,36(6):189-14. |
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| 作者姓名： | 王财金 弟文静 马淑梅 王洋 |
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| 作者单位： | 黑龙江大学现代农业与生态环境学院,150080,黑龙江哈尔滨 |
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| 基金项目： | 农业农村部作物种质资源保护(2018NWB036-12) |
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| 摘 要： | 通过发掘大豆资源中抗灰斑病1号生理小种的优异等位变异和载体材料,为开展抗灰斑病品种分子设计育种提供理论基础。以205份大豆资源构建的自然群体为试验材料,对其进行灰斑病1号生理小种的抗性鉴定;利用117对SSR标记进行全基因组扫描,分析群体的遗传多样性和群体结构,应用GLM和MLM程序对标记与大豆灰斑病抗性开展关联分析。结果表明：205份大豆资源对灰斑病1号生理小种抗性遗传变异系数为20.90%;2个模型共检测到与灰斑病1号生理小种抗性关联的位点7个,表型变异解释率在7.58%~16.06%;发掘到增效等位变异36个,其中效应值较大的等位变异为Satt244-230（26.16）、Satt142-154（21.94）和Satt244-186（20.19）,携带上述3个等位变异的载体材料均为野生资源,3个典型材料分别为12C8646、12C8670和12C6175;育成品种中具有最大增效值的等位变异为Satt142-189（8.94）,有7个品种携带该等位片段,均为黑龙江品种,典型载体材料为东农43。 上述信息可用于分子标记辅助选择育种和抗源筛选。
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| 关 键 词： | 大豆资源 灰斑病 1号生理小种 抗性 优异等位变异 |
| 收稿时间： | 2020-04-25 |
Mining the Elite Allele of Resistance of Cercospora sojina Hara Race 1 in Soybean Resources |
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| Wang Caijin,Di Wenjing,Ma Shumei,Wang Yang.Mining the Elite Allele of Resistance of Cercospora sojina Hara Race 1 in Soybean Resources[J].Crops,2020,36(6):189-14. |
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| Authors: | Wang Caijin Di Wenjing Ma Shumei Wang Yang |
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| Institution: | Department of Modern Agriculture and Ecological Environment,Heilongjiang University, Harbin 150080, Heilongjiang, China |
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| Abstract: | In order to provide theoretical basis for molecular breeding of resistance varieties by mining the elite allele and carrier materials of Cercospora sojina Hara race 1 in soybean resources, the natural population composed of 205 soybean resources was to identify the disease resistance for Cercospora sojina Hara race 1 under the artificial condition. Population structure and genetic diversity was estimated on the basis of 117 simple sequence repeat (SSR) markers. Association mapping for soybean resistance was performed by GLM model and MLM model. The results showed that the value of coefficient of variation for the resistance in 205 resources was 20.90%. We identified 7 marker-trait associations which explained variance from 7.58% to 16.06%. Thirty-six positive alleles were revealed. Satt244-230 (26.16), Satt142-154 (21.94) and Satt244-186 (20.19) had the highest positive phenotypic effect, and their carrier varieties were 12C8646, 12C8670 and 12C6175, which were all wild resources. Satt142-189 (8.94) had the highest positive phenotypic effect in varieties, which was carried by seven varieties breeding in Heilongjiang province and the typical carrier material was Dongnong 43. The above information could be used for marker-assisted selection breeding and anti-source screening. |
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| Keywords: | Soybean resources Cercospora sojina Hara Race 1 Resistance Elite allele |
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