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| 谷子抵御白发病菌侵染的生理生化及基因表达分析 | |
| 引用本文： | 韩彦卿,王鹤,王慧娜,程富英,田娜娜,闫鑫,韩渊怀.谷子抵御白发病菌侵染的生理生化及基因表达分析[J].植物病理学报,2020,50(6):657-665. |
| 作者姓名： | 韩彦卿 王鹤 王慧娜 程富英 田娜娜 闫鑫 韩渊怀 |
| 作者单位： | 山西农业大学农学院,太谷 030801; 农业生物工程研究所,太谷 030801 |
| 基金项目： | 国家自然科学基金(31701440);山西省青年科技研究基金(201701D221183);山西省重点研发项目(201703D211008);山西农业大学科技创新基金(2016YJ06) |
| 摘 要： | 白发病已成为我国谷子生产上的重要病害,不仅造成产量下降,还对谷子品质构成威胁。有关谷子品种响应白发病菌侵染的生理生化机制研究尚未见报道。本研究采用病菌卵孢子和谷粒混合的接菌方法分别接种抗病品种‘G1'和高感品种‘晋谷21'(JG21),并测定抗感谷子的植株形态指标、光合参数、防御酶活性、抗氧化酶活性、可溶性糖及脯氨酸含量,同时结合基因表达分析,旨在明确谷子响应白发病菌侵染机制。结果表明,白发病菌侵染抗感品种后,植株高度及叶面积均表现出不同程度的下降,感病品种的株高降低幅度远大于抗病品种;与抗病品种相比,感病品种中除了胞间CO2浓度显著升高外,其他光合参数显著下降,且均低于抗病品种。抗病品种中抗氧化酶和防御酶活性及脯氨酸、可溶性糖含量均升高,显著高于感病品种。抗病相关基因Seita.2G024600(PR1)、Seita.7G168700(PAL)、Seita.1G240200(PAL)和 Seita.9G462500(POD)在抗病品种中上调,而在感病品种中表达受到抑制或下调。以上光合参数、酶活性、Pro和可溶性糖含量变化等生理生化指标可作为抗性鉴定和种质筛选的参考,这些差异表达基因可能参与了谷子对白发病菌抗病调控。研究结果可为谷子抗病分子机制及分子改良育种提供理论基础。 |
| 关 键 词： | 谷子 白发病菌 防御酶 基因表达 生理生化 |
| 收稿时间： | 2020-01-15 |
Physiological,biochemical and gene expression analysis of foxtail millet against infection of Sclerospora graminicola |
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| HAN Yan-qing,WANG He,WANG Hui-na,CHENG Fu-ying,TIAN Na-na,YAN Xin,HAN Yuan-huai.Physiological,biochemical and gene expression analysis of foxtail millet against infection of Sclerospora graminicola[J].Acta Phytopathologica Sinica,2020,50(6):657-665. | |
| Authors: | HAN Yan-qing WANG He WANG Hui-na CHENG Fu-ying TIAN Na-na YAN Xin HAN Yuan-huai |
| Institution: | College of Agriculture,Shanxi Agricultural University,Taigu 030801,China; Institute of Agricultural Bioengineer,Shanxi Agricultural University,Taigu 030801,China |
| Abstract: | In recent years, Sclerospora graminicola (Sacc.) Schroeter has emerged as an important pathogen of foxtail millet production in China.The occurrence of downy mildew disease has resulted in decreased crop yield,imposing a serious threat to the quality of foxtail millet.There are few domestic and global studies on downy mildew of foxtail millet.The physiological and biochemical changes in foxtail millet responding to the infection of S.graminicola has not been reported.In this study,the oospore was mixed with seeds of the resistant variety 'G1'and the highly susceptible variety'Jingu 21' (JG21).The morphological indicators,photosynthetic parameters,defense enzyme activity,antioxidant enzyme activity,soluble sugar and proline content in the resistant and susceptible varieties were measured.Further,it was combined with gene expression analysis to clarify the mechanism of foxtail millet response to S.graminicolainfection.The results showed that the plant height and leaf area of the resistant and susceptible varieties decreased to various degrees after S.graminicola infection.However,the reduction in plant height and leaf area of the susceptible variety were much larger than that of the resistant variety.Except that the intercellular CO2 concentration increased significantly,other photosynthetic parameters decreased significantly,and the corresponding values were lower in the susceptible varieties than in the resistant ones.The activities of antioxidant enzymes and defense enzymes,as well as the content of proline and soluble sugar in resistant variety were increased,being significantly higher than those in susceptible variety.Four of six resistance-related genes Seita.2G024600 (PR1),Seita.9G462500(POD),Seita.7G168700 (PAL),and Seita.1G240200 (PAL) were up-regulated in resistant variety,whereas suppressed or down-regulated in susceptible variety.The above parameters can be used as reference indicators for resistance identification and germplasm screening.These differentially expressed genes may be involved in the regulation of foxtail millet resistant to S.graminicola.The results provide a theoretical basis for the molecular mechanism of the resistance and molecular breeding for varieties with resistance to the pathogen S.graminicola in foxtail millet. |
| Keywords: | foxtail millet S graminicola defense enzymes gene expression physiology and biochemistry |
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