| 绿色木霉固体发酵产孢子的条件优化 |
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| 引用本文： | 杨丹丹,王建平,马娜娜,张心青,倪建龙,潘冬梅.绿色木霉固体发酵产孢子的条件优化[J].中国农学通报,2020,36(12):111-119. |
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| 作者姓名： | 杨丹丹 王建平 马娜娜 张心青 倪建龙 潘冬梅 |
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| 作者单位： | 黄河三角洲京博化工研究院有限公司,山东滨州 256500 |
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| 基金项目： | 山东省科技发展项目“木霉代谢产物胶霉菌素生产的关键技术研究”(2012YD21024) |
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| 摘 要： | 为研究工业生产中利用廉价的原料发酵绿色木霉生产孢子的问题,以稻壳粉、麦麸及玉米粉为主要载体,对影响绿色木霉固态发酵产孢子的条件进行筛选,并通过单因素试验研究固体载体的比例、碳源、氮源、无机盐对绿色木霉固态发酵产孢子的影响。通过4因素3水平正交试验,优化出最佳培养基配方。研究发现,绿色木霉固体发酵产孢子的最佳条件为温度28℃,接种量15%,料水比1:0.70,pH值自然;最佳培养基配方为麸皮50%,稻壳粉30%,玉米粉20%,硫酸铵1%,玉米浆干粉2%、黄豆饼粉2%、碳酸钙0.5%。发酵7天,孢子量可达7.62×10 9个/g,自然晾干至水分10%以下,孢子量为1.286×10 10个/g。
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| 关 键 词： | 绿色木霉 固体发酵 孢子 正交试验 |
| 收稿时间： | 2018-12-28 |
Optimization of Solid-state Fermentation for Spores Production by Trichoderma viride |
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| Yang Dandan,Wang Jianping,Ma Nana,Zhang Xinqing,Ni Jianlong,Pan Dongmei.Optimization of Solid-state Fermentation for Spores Production by Trichoderma viride[J].Chinese Agricultural Science Bulletin,2020,36(12):111-119. |
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| Authors: | Yang Dandan Wang Jianping Ma Nana Zhang Xinqing Ni Jianlong Pan Dongmei |
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| Institution: | Chambroad Chemical Industry Research Institute Co., Ltd, Binzhou Shandong 256500 |
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| Abstract: | The paper aims to use cheap raw materials in industrial production to ferment spores of Trichoderma viride. We used rice husk, wheat bran and corn flour as solid supporter for the fermentation of T. viride. Four factors, including the proportion of solid support, carbon source, nitrogen source and mineral salt, were studied for their influence on the production of spores by T. viride fermentation. By the single factor and orthogonal experiment (four factors and three levels), we found that the optimum medium formulation was: bran 50%, rice husk 30%, corn flour 20%, ammonium sulfate 1%, corn starch 2%, soybean cake 2%, calcium carbonate 0.5%. This study also found that when the fermentation was conducted for 7 days under the inoculation amount of 15%, fermentation temperature of 28℃, and the ratio of material to water of 1:0.70, the spores production could reach 7.62×10 9/g, after being dried naturally and the water content was less than 10%, the spores production could reach 1.286×10 10/g. |
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| Keywords: | Trichoderma viride solid-state fermentation spores production orthogonal experiment |
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