| 浙江地区猪圆环病毒2型检测及遗传变异分析 |
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| 引用本文： | 徐丽华,蓝胜芝,余斌,李军星,张鹏超,李宝臣,苏菲,袁秀芳.浙江地区猪圆环病毒2型检测及遗传变异分析[J].浙江农业学报,2020,32(11):1970. |
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| 作者姓名： | 徐丽华 蓝胜芝 余斌 李军星 张鹏超 李宝臣 苏菲 袁秀芳 |
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| 作者单位： | 1.浙江省农业科学院 畜牧兽医研究所,浙江 杭州 310021; 2.浙江诗华诺倍威生物技术有限公司,浙江 杭州 310018 |
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| 基金项目： | 杭州市农业和社会发展科研主动设计项目(20190101A14) |
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| 摘 要： | 为了掌握浙江地区猪圆环病毒2型(porcine circovirus type 2,PCV2)流行病学特点和遗传变异情况,应用PCR检测方法,对2018年浙江地区猪场采集的临床疑似病料进行PCV2检测,并对部分阳性病料进行全基因扩增、克隆、序列测定和ORF2遗传进化树分析。结果显示:329份临床疑似样品中,PCV2阳性率27.4%(90/329),其中宁波地区阳性率最高,为52%;保育猪和肥育猪阳性率最高,分别为38.8%(64/165)和68.8%(11/16)。选取7份病料进行全基因组扩增,每个毒株随机挑选3个或4个阳性克隆进行序列测定,共得到22个毒株进行遗传变异分析。22株病株同源性为93.3%~99.8%,与16个参考毒株同源性为93.5%~99.8%。根据遗传进化树分析,22个毒株分别处于3个分支,其中6株PCV2a,6株PCV2b,10株PCV2d,说明目前浙江地区PCV2d是优势毒株。ORF2遗传进化树分析显示,ORF2核苷酸同源性较全基因组核苷酸同源性低, 3个分支分别处于PCV2b、PCV2d和PCV2e,预示浙江地区流行毒株可能在向PCV2e转变。本研究掌握了PCV2在浙江地区的流行动态,为浙江省PCV2疫苗株的选择和疫苗研发提供了理论依据。
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| 关 键 词： | 猪圆环病毒2型(PCV2) 全基因组 ORF2基因 流行病学调查 遗传变异分析 |
| 收稿时间： | 2020-04-04 |
Detection and genetic variation analysis of prevalent porcine circovirus type 2 strains in Zhejiang Province |
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| XU Lihua,LAN Shengzhi,YU Bin,LI Junxing,ZHANG Pengchao,LI Baochen,SU Fei,YUAN Xiufang.Detection and genetic variation analysis of prevalent porcine circovirus type 2 strains in Zhejiang Province[J].Acta Agriculturae Zhejiangensis,2020,32(11):1970. |
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| Authors: | XU Lihua LAN Shengzhi YU Bin LI Junxing ZHANG Pengchao LI Baochen SU Fei YUAN Xiufang |
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| Institution: | 1. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. EBVAC Biological Technology Co., Ltd., Hangzhou 310018, China |
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| Abstract: | To investigate molecular epidemiology and genetic variation of porcine circovirus type 2 (PCV2) in Zhejiang province,a total of 329 clinical samples collected in 2018 were subjected to PCR, and the whole genome of PCV2 from 7 positive samples were sequenced. The results demonstrated that the total positive rate of PCV2 was 27.4% (90/329), and the highest positive rate (52%) was in Ningbo district. By age, the highest positive rates were from fattening pigs and growing pigs (38.8% and 68.8%). Three or four clones of each of the seven randomly selected positive samples were sequenced, and genetic variation were analyzed with MEGA 6.0. Sequence comparison showed that the nucleotide sequence homologies among the 22 PCV2 strains were up to 93.3%~99.8%; and were up to 93.5%~99.8% compared with the 16 reference strains. The phylogenetic analysis revealed that these 22 strains belonged to 3 subtypes: PCV2a(6/22)、PCV2b(6/22) and PCV2d(10/22). It was indicated that PCV2d was the dominant subtype in Zhejiang province. The genetic variation analysis of ORF2 gene showed lower homology than the whole genome. The phylogenetic tree analysis of ORF2 showed that the strains were located in subtype of PCV2b, PCV2d and PCV2e respectively, indicating that the prevalent strains in Zhejiang may be shifting to PCV2e. This study explored the molecular epidemiology of PCV2 in Zhejiang province, and provided the basis for screening the candidate strain for the development of PCV2 vaccine. |
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| Keywords: | porcine circovirus type 2 whole genome ORF2 gene epidemiological investigation genetic variation analysis |
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