| 大曲中高产糖化酶菌株的筛选及产酶条件优化 |
| |
| 引用本文： | 刘延波,王肖行,赵志军,王慧慧,邢星月,潘春梅,孙西玉.大曲中高产糖化酶菌株的筛选及产酶条件优化[J].中国农学通报,2020,36(33):108-113. |
| |
| 作者姓名： | 刘延波 王肖行 赵志军 王慧慧 邢星月 潘春梅 孙西玉 |
| |
| 作者单位： | 1.河南牧业经济学院食品与生物工程学院（酒业学院）,郑州 450046;2.河南牧业经济学院河南省白酒风格工程技术研究中心,郑州 450046;3.河南牧业经济学院郑州市白酒酿造微生物技术重点实验室,郑州 450046;4.张弓老酒酒业有限公司,河南宁陵 476733 |
| |
| 基金项目： | 河南牧业经济学院博士科研启动资金项目“高通量测序技术分析浓香型白酒窖泥微生物群落结构”(906/53000158);河南牧业经济学院青年科研创新基金项目“白酒酿造微生物资源开发与微生物多样性研究”(XKYCXJJ2017015);河南省重大科技专项“豫酒品质提升关键技术及装备研发与产业化”(181100211400);河南牧业经济学院重点学科项目“发酵工程”(503/14020807) |
| |
| 摘 要： | 为了获得高产糖化酶的菌株并用于强化大曲的生产,以张弓老酒大曲为试材,通过透明圈法进行初筛以及测酶活法进行复筛,从中筛选了一株高产糖化酶的菌株WD11,其初始酶活为101 U/mL,然后通过16S rDNA序列同源性分析可以初步将该菌株归于芽孢杆菌属。在此基础上,又对其进行生理生化特性研究分析,结果表明甲基红试验、V.P试验、硝酸盐及鸟氨酸反应显阳性,赖氨酸呈阴性,故可以确定菌株WD11为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。后采用正交试验法进行了产酶条件优化,结果表明：在固体发酵培养基上,当以高粱面为最适碳源（质量分数2%）,以蛋白胨为最适氮源（质量分数4%）,接种量为6%时,其糖化酶活可达160 U/mL,是初始酶活的1.6倍,预期可以提高白酒的出酒率。
|
| 关 键 词： | 浓香型大曲 糖化酶 分子鉴定 单因素试验 正交优化 |
| 收稿时间： | 2019-11-15 |
The High-yield Glucoamylase Strains in Daqu: Screening and Optimization of Enzyme Production |
| |
| Liu Yanbo,Wang Xiaohang,Zhao Zhijun,Wang Huihui,Xing Xingyue,Pan Chunmei,Sun Xiyu.The High-yield Glucoamylase Strains in Daqu: Screening and Optimization of Enzyme Production[J].Chinese Agricultural Science Bulletin,2020,36(33):108-113. |
| |
| Authors: | Liu Yanbo Wang Xiaohang Zhao Zhijun Wang Huihui Xing Xingyue Pan Chunmei Sun Xiyu |
| |
| Institution: | 1.College of Food and Biological Engineering (Liquor College), Henan University of Animal Husbandry and Economy, Zhengzhou 450046;2.Henan Liquor Style Engineering Technology Research Center, Henan University of Animal Husbandry and Economy, Zhengzhou 450046;3.Zhengzhou Key Laboratory of Liquor Brewing Microbial Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046;4.ZhangGongLaoJiu Wine Co., Ltd., Ningling Henan 476733 |
| |
| Abstract: | To obtain the strains with high-production glucoamylase and strengthen the production of Daqu, with Zhang Gong Liquor Daqu as test material, a strain WD11 with high-production glucoamylase was screened initially with the transparent circle method and re-screened through measuring enzyme activity method. The initial enzyme activity was 101 U/mL, and the strain was preliminarily determined and belonged to bacillus genus by 16S rDNA sequence homology analysis. Based on the above, the strain was also studied by the physiological and biochemical characteristics analysis. The results showed that methyl red test, V.P test, nitrate and ornithine reaction presented positive, lysine showed negative. Therefore, WD11 strain was identified as Bacillus amyloliquefaciens. After that, the conditions of enzyme production were optimized by orthogonal experiment. The results showed that, on the solid fermentation medium, with the sorghum flour as the optimal carbon sources (mass fraction 2%), peptone as the optimal nitrogen source (mass fraction 4%), and the inoculated quantity of 6%, the saccharifying enzyme activity was up to 160 U/mL, which was1.6 times that of the initial enzyme activity. The strain is expected to increase the liquor yield. |
| |
| Keywords: | Luzhou-flavor Daqu glucoamylase molecular identification single factor test orthogonal optimization |
|
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
