| 吖啶酯偶联单克隆抗体建立化学发光法检测黄曲霉毒素B1 |
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| 引用本文： | 裘雪梅,朱立鑫,杨妮,刘云祎,刘仁荣.吖啶酯偶联单克隆抗体建立化学发光法检测黄曲霉毒素B1[J].畜牧与饲料科学,2020,41(6):78-83. |
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| 作者姓名： | 裘雪梅 朱立鑫 杨妮 刘云祎 刘仁荣 |
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| 作者单位： | 江西科技师范大学生命科学学院,江西 南昌 330013;江西科技师范大学生命科学学院,江西 南昌 330013;江西科技师范大学生命科学学院,江西 南昌 330013;江西科技师范大学生命科学学院,江西 南昌 330013;江西科技师范大学生命科学学院,江西 南昌 330013 |
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| 基金项目： | 国家自然科学基金项目“基于荧光标记模拟肽内标的黄曲霉毒素柱容量实时监测IAC-HPLC分析方法研究”（31960501） |
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| 摘 要： | 目的] 建立快速检测黄曲霉毒素B1（aflatoxin B1,AFB1）的方法。方法] 将吖啶酯（acridinium ester,AE）与AFB1单克隆抗体进行体外化学合成,分别合成了吖啶酯∶AFB1单克隆抗体摩尔比为5∶1、10∶1、15∶1、20∶1、25∶1的吖啶酯标记AFB1单克隆抗体（AFB1-AE）,分析了不同摩尔比的检测效果,选择吖啶酯∶AFB1单克隆抗体为25∶1的AFB1-AE偶联物建立化学发光免疫分析法（chemiluminescence immunoassay,CLIA）。结果] 获得对应的标准曲线回归方程为：y=-0.245Ln（x）+ 1.578 1,R2=0.985 7,IC50=81.48 pg/mL;对样品进行加标回收实验,加标回收率88.4%~106.0%,变异系数0.92%~2.10%。结论] 将吖啶酯与AFB1单克隆抗体体外交联,可建立新的化学发光免疫分析方法。
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| 关 键 词： | 黄曲霉毒素B1 吖啶酯 化学发光免疫分析法 偶联 |
| 收稿时间： | 2020-04-08 |
Detection of Aflatoxin B1 with the Chemiluminescence Method Established by Acridinium Ester Coupling with Monoclonal Antibody |
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| QIU Xue-mei,ZHU Li-xin,YANG Ni,LIU Yun-yi,LIU Ren-rong.Detection of Aflatoxin B1 with the Chemiluminescence Method Established by Acridinium Ester Coupling with Monoclonal Antibody[J].Animal Husbandry and Feed Science,2020,41(6):78-83. |
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| Authors: | QIU Xue-mei ZHU Li-xin YANG Ni LIU Yun-yi LIU Ren-rong |
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| Institution: | The Life Science College,Jiangxi Science and Technology Normal University,Nanchang 330013,China |
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| Abstract: | Objective] To establish a rapid detection method for aflatoxin B1 （AFB1）. Method] The acridinium ester （AE） and AFB1 monoclonal antibody were chemically synthesized in vitro, and the AE labeled AFB1 monoclonal antibody （AFB1-AE） with different molar ratios of AE and AFB1 monoclonal antibody of 5∶1, 10∶1, 15∶1, 20∶1 and 25∶1 was synthesized, respectively; the detection effects of AFB1-AE conjugates with different molar ratios were analyzed, and the conjugate with molar ratio of 25∶1 of AE and AFB1 monoclonal antibody was finally selected to establish chemiluminescence immunoassay （CLIA）. Result] The corresponding regression line equation was obtained: y=-0.245Ln（x） + 1.578 1, R2=0.985 7, IC50=81.48 pg/mL; the sample recovery experiment was carried out, and the standard recovery rate was 88.4%-106.0%, the coefficient of variation was 0.92%-2.10%. Conclusion] In vitro cross-linking of AE with AFB1 monoclonal antibody can be used to establish a new chemiluminescence immunoassay method. |
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| Keywords: | aflatoxin B1 acridinium ester chemiluminescence immunoassay coupling |
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