| 罗氏沼虾豚鼠气单胞菌的分离鉴定 |
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| 引用本文： | 郭莹,周淼,李艳和,王卫民.罗氏沼虾豚鼠气单胞菌的分离鉴定[J].中国农学通报,2020,36(33):147-153. |
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| 作者姓名： | 郭莹 周淼 李艳和 王卫民 |
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| 作者单位： | 华中农业大学水产学院,农业动物遗传育种与繁殖教育部重点实验室,武汉 430070 |
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| 基金项目： | 国家自然科学基金青年基金项目“基于GBS技术的克氏原螯虾系统地理学研究”(31501858) |
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| 摘 要： | 为了判断导致罗氏沼虾(Macrobrachium rosenbergii)发病死亡的原因,本研究从发病濒死的罗氏沼虾中,分离了一株具有生长优势的菌株,对分离菌株的溶血性、革兰氏染色、形态学、生理生化进行鉴定。结果表明,该分离菌株为革兰氏阴性,氧化酶、阿拉伯糖、葡萄糖产酸、蛋白胨水、精氨酸水解酶、明胶、苯丙氨酸为阳性,并对该分离菌株16S rRNA基因进行了测序以及系统进化树分析,该分离菌株与豚鼠气单胞菌聚为一支,其亲缘关系较近,综合该分离菌株理化性质和序列分析结果,可判断该分离菌株为豚鼠气单胞菌（Aeromonas caviae,登录号MN736843）。以注射法进行人工感染实验,证明了该分离菌株对罗氏沼虾具有较强的致病性,药物敏感试验结果表明,该菌株对头孢曲松、氯霉素、氟苯尼考、四环素、多西环素5种药物高度敏感,对卡那霉素1种药物中度敏感,对环丙沙星、诺氟沙星、红霉素、阿奇霉素、新生霉素、链霉素、复方新诺明和利福平8种药物耐药。本研究实验结果为罗氏沼虾豚鼠气单胞菌的病原鉴定及药物诊治提供一定的参考和理论基础。
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| 关 键 词： | 罗氏沼虾 豚鼠气单胞菌 分离鉴定 革兰氏染色 生化鉴定 药敏试验 |
| 收稿时间： | 2019-12-08 |
Aeromonas caviae from Macrobrachium rosenbergii: Isolation and Identification |
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| Guo Ying,Zhou Miao,Li Yanhe,Wang Weimin.Aeromonas caviae from Macrobrachium rosenbergii: Isolation and Identification[J].Chinese Agricultural Science Bulletin,2020,36(33):147-153. |
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| Authors: | Guo Ying Zhou Miao Li Yanhe Wang Weimin |
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| Institution: | Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070 |
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| Abstract: | To identify the mortality cause of Macrobrachium rosenbergii, the strain with growth advantage was isolated from the dying M. rosenbergii, and its hemolysis, gram staining, morphological characteristics, physiology and biochemistry were determined. The results showed that, the isolated strain was a gram-negative while oxidase, l-arabinose, glucose (acid), peptone water, arginine dihydrolase, gelatin, and phenylalanine were positive. The 16S rRNA gene of this isolate was sequenced and the phylogenetic tree was constructed. The isolated strain was clustered with Aeromonas caviae and had a close genetic relationship. The isolated strain was confirmed as Aeromonas caviae (Accession number MN736843) based on its biochemical characters and 16S rRNA sequence analysis. The artificial infection experiment with the injection method confirmed that the isolated strain was highly pathogenetic to M. rosenbergii. The results of drug susceptibility tests revealed that the strain was highly sensitive to ceftriaxone, chloramphenicol, florfenicol, tetracycline and doxycycline; it was moderately sensitivity to kanamycin and resistant to ciprofloxacin, norfloxacin, erythromycin, azithromycin, novobiocin, streptomycin, compound sulfamethoxazole and rifampicin. This study provides a reference and theoretical basis for the pathogen identification, drug diagnosis and treatment of Aeromonas cavia in M. rosenbergii. |
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| Keywords: | Macrobrachium rosenbergii Aeromonas caviae isolation and identification gram staining biochemical identification drug sensitivity test |
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