| MD疫苗免疫雏鸡vMDV攻击后体液免疫球蛋白含量的变化 |
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| 引用本文： | 高荣,刘忠贵.MD疫苗免疫雏鸡vMDV攻击后体液免疫球蛋白含量的变化[J].中国兽医杂志,1995,21(9):3-5. |
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| 作者姓名： | 高荣 刘忠贵 |
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| 作者单位： | 东北农业大学动物医学系 |
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| 摘 要： | 本实验以马立克氏病（ＭＤ）三价苗（ＳＢ＿１＋８１４＋ＨＶＴ）和ＨＶＴ疫苗免疫１日龄肉用雏鸡，１５日龄时腹腔注射马立克氏病强毒（ｖＤＭＶ）进行攻击，在攻毒后５、２５、４５和７５天用间接ＥＬＩＳＡ法测定实验雏鸡血清、泪液、气管液、胆汁和肠液ＩｇＧ、ＩｇＭ和ｌｇＡ含量的动态变化，结果发现免疫雏鸡强毒攻击后，其血清、泪液和气管液的ＩｇＧ、ｌｇＭ和ＩｇＡ含量显著高于感染对照鸡，胆汁和肠液中ＩｇＡ以及ＩｇＧ、ＩｇＭ显著高于感染对照鸡，其中三价菌免疫鸡的３种免疫球蛋白含量明显高于ＨＶＴ免疫鸡；表明ＭＤ强毒攻击后，免疫雏鸡全身和消化道、呼吸道局部的体液免疫应答显著增强，三价苗免疫鸡的体液免疫反应明显高于ＨＶＴ免疫鸡，并与其较高的免疫保护率相关。
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| 关 键 词： | 雏鸡，MD疫苗免疫，MD强毒，免疫球蛋白 |
Changes of the Content of Humoral Immunoglobulins of MD Vaccine immunized Chicks Challenged with Virulent MD Virus |
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| Gao Rong,Liu Zhonggui.Changes of the Content of Humoral Immunoglobulins of MD Vaccine immunized Chicks Challenged with Virulent MD Virus[J].Chinese Journal of Veterinary Medicine,1995,21(9):3-5. |
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| Authors: | Gao Rong Liu Zhonggui |
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| Abstract: | day-old broiler chicks were inoculated respectively with trivalent(SB_1 814 HVT)andHVT Marek's disease vaccine, and challeged with virulent MD virus at the age of 15 days.5, 25, 45 and 75days postchallenge the experimental chicks were taken to collect serum ,tear,trachea washings dile and in-testinal flaid to detect the dynamic changes of the cotent of lgG, IgM and lgA by application of indirect en-zyme-Linked immunoassay test. The results showed that after challenge with vDMV, IgG, IgM and IgAsigni-ficantly increased in the serum,tear and trachea washings of the immunized chicks in comparison withthose of the infected Control chicks;the content of IgA,IgG and IgM of the immunized birds were also marke-ly higher than those of the infected control group in the bile and intestinal fluid;the content of the im-munoglobulins in the mentioned above humore of the trivalent vaccine-immunized chicks was significantlybigher than that of the HVT-immunized chicks,the immunoprotectiOn of trivalent vaccine-immunizedchicks against challenge was better than that of HVT-vaccinated chicks.Tberesults suggest that the sys-temic and local humoral immune response in the digestire and respiratory tract was sigificantly enhanced inthe vaccinated chicks after challenge with vDMV,the stronger humoral immune response was elicited in thechicks inoculated with trivalent vaccine,which was closely related with the higher immunoprotective efficacy. |
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| Keywords: | chick MD vaccine immunization virulent MD virus Immunoglobulins |
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