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Application of microparticle diets for Japanese flounder Paralichthys olivaceus larvae
Authors:Qiurong  WANG  Toshio  TAKEUCHI  Tetsuya  HIROTA  Shuzo  ISHIDA  Hiroshi  MIYAKAWA AND Hirotoshi  HAYASAWA
Institution:Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477;,
Taiyo Yushi KK, Kanagawa, Yokohama, Kanagawa 221-0022;and
Morinaga Milk Industry, Zama, Kanagawa 228-8583, Japan
Abstract:ABSTRACT:   A feeding trial was conducted with two newly developed microparticle diets (MD-Q and MD-V), which differed in the mixing ratios of two types of casein hydrolysates (C700 and C800) as the protein source. The ratio of C700 and C800 were adjusted to 7:3 in MD-Q and 6:4 in MD-V, respectively. Japanese flounder larvae were fed from 7 days after hatching (d.a.h.) to 32 d.a.h. on live food (LF), MD solely, MD + 1/3 LF (either of the MD and one-third quantity of the live food) and 1/3 LF (one-third quantity of live food alone), respectively. They were then switched to Kyowa diet B from 33 d.a.h. until 40 d.a.h. The larvae fed on MD-Q exclusively had a higher survival rate (36.4%) than those fed on MD-V (24.2%) by 22 d.a.h. Unfed larvae could survive up to only 12 d.a.h. Larvae fed on MD-Q + 1/3 LF also had a significantly higher survival rate (34.2%) than those on MD-V + 1/3 LF (16.3%) and 1/3 LF solely (15.4%) at 32 d.a.h. These results suggest the potential of MD-Q as a part replacement for LF from the early developmental stage in the seed production of Japanese flounder.
Keywords:Japanese flounder  larvae  microparticle diet  peptide
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